TLR7 Modulated T Cell Response in the Mesenteric Lymph Node of Schistosoma japonicum-Infected C57BL/6 Mice.

Abstract

Toll-like receptors (TLRs) play an important role in regulating immune responses during pathogen infection. However, roles of TLRs on T cells reside in the mesenteric lymph node (MLN) were not be fully elucidated in the course of S. japonicum infection. In this study, T lymphocytes from the mesenteric lymph node (MLN) of S. japonicum-infected mice were isolated and the expression and roles of TLR2, TLR3, TLR4, and TLR7 on both CD4+ and CD8+ T cells were compared. We found that the expression of TLR7 was increased in the MLN cells of S. japonicum-infected mice, particularly in CD4+ and CD8+ T cells (P < 0.05). R848, a TLR7 agonist, could enhance the production of IFN-γ from MLN T cells of infected mice (P < 0.05), especially in CD8+ T cells (P < 0.01). In TLR7 gene knockedout (KO) mice, the S. japonicum infection caused a significant decrease (P < 0.05) of the expression of CD25 and CD69, as well as the production of IFN-γ and IL-4 inducted by PMA plus ionomycin on both CD4+ and CD8+ T cells. Furthermore, the decreased level of IFN-γ and IL-4 in the supernatants of SEA- or SWA-stimulated mesenteric lymphocytes was detected (P < 0.05). Our results indicated that S. japonicum infection could induce the TLR7 expression on T cells in the MLN of C57BL/6 mice, and TLR7 mediates T cell response in the early phase of infection.

• Authors: Jiale Qu, Xiuxue Yu, Chenxi Jin, Yuanfa Feng, Shihao Xie, Hongyan Xie, Quan Yang, Yanwei Qi, Huaina Qiu, Hongyuan Chen, Jianbing Mu, Yi Zhou, Jun Huang
• Journal: Journal of immunology research (J Immunol Res) Vol. 2019 Pg. 2691808 ( 2019) ISSN: 2314-7156 [Electronic] Egypt
• PMID: 31930147 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 Jiale Qu et al.

Chemical References

• Antigens, CD
• Antigens, Differentiation, T-Lymphocyte
• CD69 antigen
• Il2ra protein, mouse
• Imidazoles
• Interleukin-2 Receptor alpha Subunit
• Lectins, C-Type
• Toll-Like Receptor 7
• Interleukin-4
• Interferon-gamma
• resiquimod

Topics

• Animals
• Antigens, CD (immunology)
• Antigens, Differentiation, T-Lymphocyte (immunology)
• CD4-Positive T-Lymphocytes (cytology, immunology, metabolism)
• CD8-Positive T-Lymphocytes (cytology, immunology, metabolism)
• Female
• Imidazoles (pharmacology)
• Interferon-gamma (metabolism)
• Interleukin-2 Receptor alpha Subunit (immunology)
• Interleukin-4 (metabolism)
• Lectins, C-Type (immunology)
• Lymph Nodes (cytology, metabolism)
• Mesentery
• Mice
• Mice, Inbred C57BL
• Mice, Knockout
• Schistosomiasis japonica (immunology)
• Toll-Like Receptor 7 (agonists, genetics, metabolism)