25-Hydroxycholesterol protects against myocardial ischemia-reperfusion injury via inhibiting PARP activity.

Abstract

Myocardial ischemia-reperfusion (IR) injury occurs when occlusive coronary artery restores blood supply after events such as myocardial infarction, stroke, cardiac arrest and resuscitation, and organ transplantation. However, the mechanisms involved are poorly understood, and effective pharmacological interventions are still lacking. A previous study demonstrated that 25-hydroxycholesterol (25-HC) contributed to lipid metabolism and cholesterol metabolism as an oxysterol molecule. We herein explored whether 25-hydroxycholesterol (25-HC) has cardioprotective properties against IR injury and explored its underlying mechanisms. 25-HC was administered before reperfusion procedure in IR injury model mice. We found that 25-HC significantly reduced the IR-induced infarct size and improved cardiac function, and this protective effect was associated with reduced phosphorylation of p38-MAPK and JNK1/2. Besides, 25-HC also inhibited the Bax/Bcl-2 ratio and the relative expression of cleaved caspase-3. Furthermore, 25-HC decreased the PARP activity, indicating that 25-HC ameliorates IR injury via the PARP pathway. The 25-HC group abolished cardioprotection in the presence of little PARP activity, suggesting that the PARP activity is essential for 25-HC to exert its effect during IR injury. Our primary study indicates that 25-HC ameliorated IR injury by inhibiting the PARP activity and decreasing myocardial apoptosis, which makes it a potential therapeutic drug in IR injury of the heart.

• Authors: Suying Lv, Chenhui Ju, Jiangtong Peng, Minglu Liang, Feng Zhu, Cheng Wang, Kai Huang, Min Cheng, Fengxiao Zhang
• Journal: International journal of biological sciences (Int J Biol Sci) Vol. 16 Issue 2 Pg. 298-308 ( 2020) ISSN: 1449-2288 [Electronic] Australia
• PMID: 31929757 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © The author(s).

Chemical References

• Hydroxycholesterols
• 25-hydroxycholesterol
• p38 Mitogen-Activated Protein Kinases

Topics

• Animals
• Apoptosis (drug effects)
• Blotting, Western
• Cells, Cultured
• Echocardiography
• Hydroxycholesterols (therapeutic use)
• In Situ Nick-End Labeling
• Male
• Mice
• Mice, Inbred C57BL
• Myocardial Reperfusion Injury (drug therapy, metabolism)
• Myocardium (metabolism)
• Oxidative Stress (drug effects)
• Phosphorylation (drug effects)
• Signal Transduction (drug effects)
• p38 Mitogen-Activated Protein Kinases (metabolism)