Abstract |
Exposure to combustion-derived nanoparticles is recognized as a major health hazard, but the molecular responses are still insufficiently described. The transcription factor erythroid 2-related factor 2 (Nrf2, also known as NFE2L2) is a master regulator of the pulmonary defense system against insults by particulate matter. However, its downstream molecular processes are not fully characterized. In the current study, BALB/c wild-type (WT) and Nrf2-/- mice were exposed by intranasal administration to fly ash particles (F3-S; 20 mg/kg BW), which were collected from a municipal waste incinerator in China, for three consecutive days. Using a comparative transcriptomics approach, the pulmonary global gene expression profiles to F3-S exposure were characterized for both genotypes. The preponderance of the differentially-expressed genes (DEGs) in WT mice induced by the fly ash particles, was related to inflammation. Functional enrichment and molecular pathway mapping of the DEGs specific to Nrf2-/- mice exposed to the particles revealed that all of the top 10 perturbed molecular pathways were associated with the inflammatory response. Our study identified a transcriptional signature related to the initial pulmonary injury in mouse upon fly ash exposure, and suggests an anti-inflammatory role of Nrf2 in protecting the lung against such exposure.
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Authors | Jingwen Zhang, Huiling Cui, Akhileshwar Namani, Jun Yao, Hong Deng, Xiuwen Tang, Xiu Jun Wang |
Journal | Ecotoxicology and environmental safety
(Ecotoxicol Environ Saf)
Vol. 190
Pg. 110132
(Mar 01 2020)
ISSN: 1090-2414 [Electronic] Netherlands |
PMID | 31918253
(Publication Type: Journal Article)
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Copyright | Copyright © 2019 Elsevier Inc. All rights reserved. |
Chemical References |
- Coal Ash
- NF-E2-Related Factor 2
- Particulate Matter
- Carbon
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Topics |
- Animals
- Carbon
- China
- Coal Ash
(analysis)
- Gene Expression Profiling
- Incineration
- Lung
(chemistry)
- Mice
- Mice, Inbred BALB C
- NF-E2-Related Factor 2
(metabolism)
- Particulate Matter
(toxicity)
- Transcriptome
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