Abstract | INTRODUCTION: The mechanism of fast inspiratory flow rate (VI') induced lung injury is unclear. As fast VI' increases hysteresis, a measure of surface tension at the air-liquid interface, surfactant release or function may be important. This experimental study examines the contribution of impaired surfactant release or function to dynamic-VILI. METHODS: Isolated perfused lungs from male Sprague Dawley rats were randomly allocated to four groups: a long or short inspiratory time (Ti = 0.5 s; slow VI' or Ti = 0.1 s; fast VI') at PEEP of 2 or 10 cmH2O. Tidal volume was constant (7 ml/kg), with f = 60 breath/min. Forced impedance mechanics (tissue elastance (Htis), tissue resistance (Gtis) and airway resistance (Raw) were measured at 30, 60 and 90 min following which the lung was lavaged for surfactant phospholipids (PL) and disaturated PL (DSP). RESULTS: Fast VI' resulted in a stiffer lung. Concurrently, PL and DSP were decreased in both tubular myelin rich and poor fractions. Phospholipid decreases were similar with PEEP. In a subsequent cohort, laser confocal microscopy-based assessment demonstrated increased cellular injury with increased VI' at both 30 and 90 min ventilation. CONCLUSION:
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Authors | Andrew D Bersten, Malgorzata Krupa, Kim Griggs, Dani-Louise Dixon |
Journal | Lung
(Lung)
Vol. 198
Issue 1
Pg. 43-52
(02 2020)
ISSN: 1432-1750 [Electronic] United States |
PMID | 31915922
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cytokines
- Phospholipids
- Pulmonary Surfactants
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Topics |
- Airway Resistance
(physiology)
- Animals
- Cytokines
(metabolism)
- Elasticity
- Lung
(metabolism, pathology, physiopathology)
- Lung Compliance
(physiology)
- Phospholipids
(metabolism)
- Positive-Pressure Respiration
- Pulmonary Surfactants
(metabolism)
- Random Allocation
- Rats
- Respiration, Artificial
- Respiratory Mechanics
(physiology)
- Tidal Volume
- Ventilator-Induced Lung Injury
(metabolism, pathology, physiopathology)
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