A cancer-specific activatable theranostic nanodrug for enhanced therapeutic efficacy via amplification of oxidative stress.

Abstract

Rationale: Despite considerable advances, the reactive oxygen species (ROS)-mediated cancer treatment suffers from the problems of up-regulation of adaptive antioxidants in cancer cells as well as side effects to normal cells. Therefore, development of a new generation of cancer-specific nanomedicine capable of amplifying oxidative stress would be of great interest for accurate and effective cancer treatment. Methods: Herein, transferrin (Tf)-decorated, dihydroartemisinin (DHA), L-buthionine-sulfoximine (BSO), and CellROX-loaded liposomal nanoparticles (Tf-DBC NPs) were developed for precise cancer theranositcs. Tf-DBC NPs could specifically recognize cancer cells via Tf-Tf receptor binding and be uptaken into the lysosomes of cancer cells, where Tf-DBC NPs were activated to release Fe(II), DHA, and BSO. ROS was generated by DHA in the presence of Fe(II), and GSH was depleted by BSO to disrupt the redox balance in cancer cells. Furthermore, CellROX, as a fluorescent probe for imaging of intracellular oxidative stress, was used to monitor the therapeutic efficacy. Results: The integration of Tf, DHA, and BSO into the acidic pH-responsive liposomes selectively and effectively killed cancer cells and prevented the oxidative injury to normal cells. The high oxidative state was visualized at the tumor site and the amplification of oxidative stress enabled tumor eradication by Tf-DBC NPs, demonstrating the successful implementation of this novel strategy in vivo. Conclusion: Our study provides a new paradigm for the design of ROS-mediated therapeutics and offers a promising perspective for precise cancer treatment.

• Authors: Xie-An Yu, Mi Lu, Yingping Luo, Yiting Hu, Ying Zhang, Zhiming Xu, Shuaishuai Gong, Yunhao Wu, Xiao-Nan Ma, Bo-Yang Yu, Jiangwei Tian
• Journal: Theranostics (Theranostics) Vol. 10 Issue 1 Pg. 371-383 ( 2020) ISSN: 1838-7640 [Electronic] Australia
• PMID: 31903126 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © The author(s).

Chemical References

• Artemisinins
• Drug Carriers
• Liposomes
• Reactive Oxygen Species
• Transferrin
• Buthionine Sulfoximine
• artenimol
• Glutathione

Topics

• Animals
• Artemisinins (pharmacology, therapeutic use)
• Buthionine Sulfoximine (pharmacology, therapeutic use)
• Drug Carriers (chemistry)
• Female
• Glutathione (metabolism)
• Hep G2 Cells
• Humans
• Liposomes (chemistry)
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Nanoparticles (chemistry)
• Neoplasms (therapy)
• Oxidation-Reduction (drug effects)
• Oxidative Stress (drug effects)
• Precision Medicine
• Reactive Oxygen Species (metabolism)
• Transferrin (chemistry)