Abstract | SCOPE: METHODS AND RESULTS: Two-week-old male wild-type (WT) and BCO1-/- /BCO2-/- double knockout (DKO) mice are given a single intraperitoneal injection of DEN (25 mg kg-1 body weight) to initiate hepatic carcinogenesis. At 6 weeks of age, all animals are fed HRCD (66.5% of energy from carbohydrate) with or without BCX for 24 weeks. BCX feeding increases hepatic vitamin A levels in WT mice, but not in DKO mice that shows a significant accumulation of hepatic BCX. Compared to their respective HRCD littermates, both WT and DKO fed BCX have significantly lower HCC multiplicity, average tumor size, and total tumor volume, and the steatosis scores. The chemopreventive effects of BCX are associated with increased p53 protein acetylation and decreased protein levels of lactate dehydrogenase and hypoxia-inducible factor-1α in tumors. CONCLUSION: This study suggests that BCX feeding may alleviate HRCD-promoted HCC progression by modulating the acetylation of p53, hypoxic tumor microenvironment, and glucose metabolism, independent of BCO1/BCO2.
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Authors | Ji Ye Lim, Chun Liu, Kang-Quan Hu, Donald E Smith, Dayong Wu, Stefania Lamon-Fava, Lynne M Ausman, Xiang-Dong Wang |
Journal | Molecular nutrition & food research
(Mol Nutr Food Res)
Vol. 64
Issue 3
Pg. e1900949
(02 2020)
ISSN: 1613-4133 [Electronic] Germany |
PMID | 31891208
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
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Copyright | © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Chemical References |
- Beta-Cryptoxanthin
- Dietary Carbohydrates
- Diterpenes
- Hif1a protein, mouse
- Hypoxia-Inducible Factor 1, alpha Subunit
- Retinyl Esters
- Trp53 protein, mouse
- Tumor Suppressor Protein p53
- Vitamin A
- retinol palmitate
- Dioxygenases
- Bco1 protein, mouse
- Bco2 protein, mouse
- beta-Carotene 15,15'-Monooxygenase
- Glucose
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Topics |
- Animals
- Apoptosis
(drug effects)
- Beta-Cryptoxanthin
(pharmacology)
- Body Weight
(drug effects)
- Carcinoma, Hepatocellular
(drug therapy, etiology, pathology)
- Dietary Carbohydrates
(adverse effects)
- Dietary Supplements
- Dioxygenases
(genetics)
- Diterpenes
(analysis)
- Glucose
(metabolism)
- Glycolysis
(drug effects)
- Hypoxia-Inducible Factor 1, alpha Subunit
(metabolism)
- Liver Neoplasms
(drug therapy, etiology, pathology)
- Male
- Mice, Inbred C57BL
- Mice, Knockout
- Retinyl Esters
(analysis)
- Tumor Hypoxia
(drug effects)
- Tumor Microenvironment
(drug effects)
- Tumor Suppressor Protein p53
(metabolism)
- Vitamin A
(analysis)
- beta-Carotene 15,15'-Monooxygenase
(genetics)
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