Changes in heparan sulfate sulfotransferases and cell-surface heparan sulfate during SKM-1 cells granulocytic differentiation and A549 cells epithelial-mesenchymal transition.

Abstract

Heparan sulfate (HS) with various sulfation patterns is one of important modulators of cancer cell fate through interacting with numerous growth factors. Here we found HS 2-O sulfotransferase 1 (HS2ST1) was downregulated at both mRNA and protein levels during granulocytic differentiation of SKM-1 leukemia cells and also HS (glucosamine) 3-O sulfotransferase 3A (HS3ST3A) in epithelial-mesenchymal transition (EMT) of A549 lung cancer cells, meanwhile, cell-surface HS recognized by anti-HS antibody was also changed in both cancer cell lines. Further, HS3ST3A was negatively correlated with the in vitro cell metastasis capability of A549 cells confirmed by RNA interference technology, wound-healing assay and in vitro Matrigel invasion assay. Together, specific HS sulfotransferases may serve as molecular markers and targets for cancer treatment.

• Authors: Shancheng Zhao, Zhen Wang
• Journal: Glycoconjugate journal (Glycoconj J) Vol. 37 Issue 2 Pg. 151-164 (04 2020) ISSN: 1573-4986 [Electronic] United States
• PMID: 31863309 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Heparitin Sulfate
• Sulfotransferases
• heparan-sulfate 2-sulfotransferase

Topics

• A549 Cells
• Cell Differentiation
• Cell Movement
• Epithelial-Mesenchymal Transition
• Granulocytes (cytology, metabolism)
• Heparitin Sulfate (metabolism)
• Humans
• Sulfotransferases (genetics, metabolism)