Abstract | AIM: METHODS: Three-day-old pups were given LPS (1250 μg/kg) or saline (controls). Liver HO enzyme activity, HO-1 mRNA and HO-1 protein were measured post-LPS exposure. We then assessed the effects of LPS treatment on in vivo bilirubin production rates after haem loading. RESULTS: Liver HO activity significantly increased (142%) over controls 24 hours after treatment with LPS (1250 μg/kg) without mortality. Liver HO-1 mRNA was significantly upregulated 2.47-fold at 24 hours post-LPS administration, while liver HO-1 protein increased 1.29-fold 24 hours post-LPS treatment. After haem loading, pups exposed to LPS had significantly higher bilirubin production rates (1.30-fold) compared with age-matched, saline-treated controls. CONCLUSION: Low-dose LPS treatment can upregulate liver HO-1 expression and may underlie the severe hyperbilirubinaemia seen in septic infants, particularly when undergoing haemolysis.
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Authors | Sota Iwatani, Danielle R Jacobsen, Ronald J Wong, David K Stevenson |
Journal | Acta paediatrica (Oslo, Norway : 1992)
(Acta Paediatr)
Vol. 109
Issue 8
Pg. 1551-1559
(08 2020)
ISSN: 1651-2227 [Electronic] Norway |
PMID | 31860732
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2019 Foundation Acta Paediatrica. Published by John Wiley & Sons Ltd. |
Chemical References |
- Lipopolysaccharides
- Heme Oxygenase (Decyclizing)
- Bilirubin
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Topics |
- Animals
- Animals, Newborn
- Bilirubin
- Heme Oxygenase (Decyclizing)
- Hyperbilirubinemia
- Lipopolysaccharides
(toxicity)
- Mice
|