Species identification, virulence markers and antimicrobial resistance profiles of Aeromonas sp. isolated from marketed hard-shelled mussel (Mytilus coruscus) in Korea.

Abstract

Hard-shelled mussel (Mytilus coruscus) is a popular seafood in Korea. This study aimed to determine the virulence markers and antimicrobial resistance patterns of 33 Aeromonas strains isolated from mussels. The isolates were identified as A. salmonicida (n = 14), A. veronii (n = 9), A. enteropelogenes (n = 4), A. caviae (n = 3), A. allosaccharophila (n = 2) and A. bivalvium (n = 1) by gyrB gene sequencing. The sequence divergence between and within the species ranged from 3·70 to 10·40% and 0-1·50% respectively. Every species formed a distinct group in a neighbour-joining phylogenetic tree. The DNase, gelatinase, caseinase, β-haemolysis, biofilm and lipase activities were observed in 33 (100·00%), 31 (93·93%), 30 (90·90%), 27 (81·81%), 21 (63·63%) and 17 (51·51%) isolates respectively. The virulence genes were detected by PCR in the following frequencies: fla (90·09%), aer (87·88%), hlyA (87·88%), ahyB (81·19%), gcaT (75·76%), ser (69·70%), lip (66·67%), alt (57·58%), ast (51·51%) and act (21·21%). Every isolate was resistant to at least three of 18 antimicrobials in the disk diffusion test. The multiple antimicrobial resistance index values ranged from 0·11 to 0·44 among the isolates. Our study suggests that mussels can be a potential reservoir of virulent and multidrug-resistant Aeromonas sp. SIGNIFICANCE AND IMPACT OF THE STUDY: Aeromonas sp. are known as common pathogenic bacteria isolated from seafood. The virulence factors and antimicrobial resistance profiles of mussel-borne Aeromonas sp. are poorly understood. This study demonstrated for the first time the existence of virulence markers and antimicrobial resistance of Aeromonas sp. from mussels in Korea. Majority of the isolates were positive for phenotypic virulence characteristics and harboured several virulence genes which reveal the potential virulence of mussel-borne Aeromonas sp. Multiple antimicrobial resistance was also observed among the isolates. Our study highlights the importance of food safety standards in mussel consumption.

• Authors: S Hossain, M V K S Wickramanayake, P S Dahanayake, G-J Heo
• Journal: Letters in applied microbiology (Lett Appl Microbiol) Vol. 70 Issue 3 Pg. 221-229 (Mar 2020) ISSN: 1472-765X [Electronic] England
• PMID: 31854000 (Publication Type: Journal Article)
• Copyright: © 2019 The Society for Applied Microbiology.

Chemical References

• Anti-Bacterial Agents
• Virulence Factors
• Acyltransferases
• Aeromonas hydrophilia lipase-acyltransferase
• Lipase

Topics

• Acyltransferases (genetics)
• Aeromonas (classification, genetics, isolation & purification, pathogenicity)
• Animals
• Anti-Bacterial Agents (pharmacology)
• Biofilms (drug effects, growth & development)
• Drug Resistance, Bacterial (genetics)
• Gram-Negative Bacterial Infections (microbiology)
• Lipase (genetics)
• Mytilus (microbiology)
• Phylogeny
• Polymerase Chain Reaction
• Republic of Korea
• Seafood (microbiology)
• Virulence (genetics)
• Virulence Factors (genetics)