Sildenafil and tadalafil reduce the risk of contrast-induced nephropathy by modulating the oxidant/antioxidant balance in a murine model.

Abstract

The aim of the study was to evaluate the potential protective role of sildenafil and tadalafil in contrast-induced nephropathy (CIN) by modulating oxidative stress. Thirty Wistar male rats were equally assigned into five groups: sham, CIN, CIN + sildenafil (10 mg/kg bw/day), CIN + tadalafil (5 mg/kg bw/day) and CIN + N-Acetyl Cysteine (NAC) (100 mg/kg bw/day) as a positive control. CIN was induced by 12 h dehydration and administration of indomethacin (10 mg/kg bw), N-ω- nitro-L-arginine methyl ester (10 mg/kg bw), and iopromide (3 g/kg bw iodine). Blood was drawn prior to and 24 h after CIN induction for evaluating renal function and oxidative stress. In the CIN group, total antioxidant capacity (TAC), reduced glutathione (GSH) and catalase (CAT) levels were significantly decreased; and protein carbonyl (PROTC) and thiobarbituric reactive species (TBARS) were significantly increased compared to the sham group. Pre- Sildenafil and tadalafil pre-treatment reduced CIN risk and reversed oxidative stress almost to the sham group levels. These results suggest that PDE5Is can be good candidates for preventing CIN based on their ability to modulate the oxidant/antioxidant balance.

• Authors: Andrei Mihai Iordache, Anca Oana Docea, Ana Maria Buga, Ovidiu Zlatian, Marius Eugen Ciurea, Otilia Constantina Rogoveanu, Florin Burada, Simona Sosoi, Radu Mitrut, Charalampos Mamoulakis, Dana Albulescu, Ramona Constantina Vasile, Aristides Tsatsakis, Daniela Calina
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 135 Pg. 111038 (Jan 2020) ISSN: 1873-6351 [Electronic] England
• PMID: 31825855 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 Elsevier Ltd. All rights reserved.

Chemical References

• Antioxidants
• Contrast Media
• Oxidants
• Phosphodiesterase 5 Inhibitors
• Thiobarbituric Acid Reactive Substances
• Tadalafil
• Sildenafil Citrate
• Catalase
• Glutathione
• Acetylcysteine

Topics

• Acetylcysteine (administration & dosage)
• Animals
• Antioxidants (metabolism)
• Catalase (metabolism)
• Contrast Media (adverse effects)
• Disease Models, Animal
• Glutathione (metabolism)
• Kidney Diseases (chemically induced, enzymology, metabolism, prevention & control)
• Male
• Mice
• Oxidants (metabolism)
• Oxidative Stress
• Phosphodiesterase 5 Inhibitors (pharmacology)
• Rats
• Rats, Wistar
• Sildenafil Citrate (pharmacology)
• Tadalafil (pharmacology)
• Thiobarbituric Acid Reactive Substances (metabolism)