Abstract | OBJECTIVE: MATERIALS AND METHODS: RESULTS:
BDNF, LXA4 and AA, EPA and DHA protected (P < 0.001 and P < 0.01 respectively) against AL/STZ/DB/BP-induced toxicity to RIN5F cells in vitro. AL/ STZ/DB/BP inhibited BDNF and LXA4 production by RIN5F cells and were restored to normal by AA, EPA and DHA. Sub-optimal doses of BDNF, LXA4, AA and EPA when used in combination protected against cytotoxic action of AL/STZ/DB/BP on RIN5F cells in vitro by restoring LXA4/ BDNF levels and altered antioxidant/ lipid peroxides/NO levels (P < 0.01) to normal. STZ (65 mg/kg)-induced type 2 diabetes mellitus animals showed reduced plasma BDNF and LXA4 levels (P < 0.001). DISCUSSION: AL/STZ/DB/BP-induced cytotoxicity to RIN5F cells in vitro can be prevented by BDNF, LXA4 and AA. AL/STZ/DB/BP are cytotoxic, possibly, by suppressing the production of LXA4 and BDNF in RIN5F cells. STZ-induced type 2 DM animals have decreased plasma levels of LXA4 and BDNF. CONCLUSION: The results of the present study suggest that BDNF, LXA4, EPA, DHA, AA, GLA and BDNF protect pancreatic β cells from the cytotoxic action of various chemicals and prevent development of diabetes mellitus. LXA4 seems to be the mediator of these cytoprotective actions of BDNF and PUFAs suggesting a close interaction exists among these molecules ( BDNF, PUFAs and LXA4). Hence, methods developed to deliver a combination of PUFAs (especially AA), LXA4 and BDNF may prevent development of diabetes mellitus (both type 1 and type 2).
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Authors | Siresha Bathina, Undurti N Das |
Journal | Lipids in health and disease
(Lipids Health Dis)
Vol. 18
Issue 1
Pg. 214
(Dec 10 2019)
ISSN: 1476-511X [Electronic] England |
PMID | 31823816
(Publication Type: Journal Article)
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Chemical References |
- Brain-Derived Neurotrophic Factor
- Cytotoxins
- Fatty Acids, Unsaturated
- Lipoxins
- lipoxin A4
- Arachidonic Acid
- Benzo(a)pyrene
- Streptozocin
- Alloxan
- Doxorubicin
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Topics |
- Alloxan
(pharmacology)
- Animals
- Arachidonic Acid
(pharmacology)
- Benzo(a)pyrene
(pharmacology)
- Brain-Derived Neurotrophic Factor
(administration & dosage, blood)
- Cell Death
(drug effects)
- Cell Line, Tumor
- Cytotoxins
- Diabetes Mellitus, Experimental
(blood, pathology)
- Diabetes Mellitus, Type 2
(blood, pathology)
- Doxorubicin
(pharmacology)
- Drug Interactions
- Fatty Acids, Unsaturated
(administration & dosage)
- Insulin-Secreting Cells
(drug effects, pathology)
- Insulinoma
- Lipoxins
(administration & dosage, blood)
- Pancreatic Neoplasms
- Rats
- Rats, Wistar
- Streptozocin
(pharmacology)
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