Prognostic Value of TWIST1 and EZH2 Expression in Colon Cancer.

Abstract

BACKGROUND:

Colorectal carcinoma (CRC) is the third most common human cancer. Twist, a basic helix-loop-helix (bHLH) transcription factor, is an epithelial-mesenchymal transition ((EMT) inducer that has been involved in carcinogenesis and chemoresistance. Also, the enhancer of Zeste homolologue2 (EZH2), a member of the polycomb group of genes, had been associated with poor prognosis in several malignancies.

OBJECTIVE:

To evaluate the expression of Twist1 and EZH2 in colon carcinoma in Egyptian patients and its relation to clinicopathological parameters, prognosis, and survival.

METHODS:

Twist1 and EZH2 expressions were evaluated immunohistochemically in 50 cases of colorectal tumors (12 colon adenomas and 38 colon carcinomas) and 20 samples from normal colonic mucosa.

RESULTS:

The expression of Twist1 and EZH2 was significantly higher in colon adenoma and carcinoma than that in normal colonic mucosa (P < 0.05). Twist1 and EZH2 expressions were associated with decreased tumor differentiation, advanced stage, and lymph node metastasis. Twist1 and EZH2 expressions were significantly related to 3-year disease-free survival (P = 0.005 and 0.002 respectively) and 3-year overall survival (P = 0.045 and 0.039, respectively).

CONCLUSIONS:

Twist1 and EZH2 may serve as prognostic predictors for colon carcinoma and may have a potential role as therapeutic targets in patients with colon carcinoma in the future.

• Authors: Samar M Abdel Raouf, Taiseer R Ibrahim, Lobna A Abdelaziz, Mohamed I Farid, Salem Y Mohamed
• Journal: Journal of gastrointestinal cancer (J Gastrointest Cancer) Vol. 52 Issue 1 Pg. 90-98 (Mar 2021) ISSN: 1941-6636 [Electronic] United States
• PMID: 31823218 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• Nuclear Proteins
• Organoplatinum Compounds
• TWIST1 protein, human
• Twist-Related Protein 1
• EZH2 protein, human
• Enhancer of Zeste Homolog 2 Protein
• Leucovorin
• Fluorouracil

Topics

• Adult
• Antineoplastic Combined Chemotherapy Protocols (therapeutic use)
• Biomarkers, Tumor (analysis, metabolism)
• Carcinoma (diagnosis, drug therapy, genetics, mortality)
• Colonic Neoplasms (diagnosis, drug therapy, genetics, mortality)
• Disease-Free Survival
• Egypt (epidemiology)
• Enhancer of Zeste Homolog 2 Protein (analysis, metabolism)
• Epithelial-Mesenchymal Transition (genetics)
• Female
• Fluorouracil (therapeutic use)
• Gene Expression Regulation, Neoplastic
• Humans
• Immunohistochemistry
• Leucovorin (therapeutic use)
• Lymphatic Metastasis (genetics)
• Male
• Middle Aged
• Neoplasm Recurrence, Local (epidemiology, genetics, pathology)
• Neoplasm Staging
• Nuclear Proteins (analysis, metabolism)
• Organoplatinum Compounds (therapeutic use)
• Prognosis
• Prospective Studies
• Risk Assessment (methods)
• Twist-Related Protein 1 (analysis, metabolism)
• Young Adult