Monoclonal antibodies to dalargin--a synthetic analog of enkephalins.

Abstract

Monoclonal antibodies (MABs) to a new drug Dalargin (Tyr-D-Ala-Gly-Phe-Leu-Arg) enhancing ulcer healing have been produced. Dalargin is a synthetic analog of Leu-enkephalin. With about 40 compounds tested in competition radioimmunoassay it has been shown that specificity of the MABs is directed against the N-terminal tetrapeptide of the molecule. The MABs are sensitive to amino acid substitutions in any of the positions of the fragment and have no cross-reactivity with endogenous opioids. Their further application in pharmacokinetic studies in humans and for characterization of opioid receptors is discussed.

• Authors: N M Ananyeva, V A Tischenko, J D Bespalova, B L Pekelis, L Z Yakubov, V A Vinogradov, O V Rokhlin, M I Titov
• Journal: Immunology letters (Immunol Lett) Vol. 18 Issue 4 Pg. 269-73 (Aug 1988) ISSN: 0165-2478 [Print] Netherlands
• PMID: 3182012 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Peptide Fragments
• Enkephalin, Leucine
• Enkephalin, Leucine-2-Alanine
• enkephalin-Leu, Ala(2)-Arg(6)-

Topics

• Amino Acid Sequence
• Animals
• Antibodies, Monoclonal
• Enkephalin, Leucine (analogs & derivatives, immunology)
• Enkephalin, Leucine-2-Alanine (analogs & derivatives)
• Male
• Mice
• Mice, Inbred BALB C
• Molecular Sequence Data
• Peptide Fragments (immunology)