Abstract | BACKGROUND: The detection of glutamic acid decarboxylase 65 (GAD65) autoantibodies is essential for the prediction and diagnosis of latent autoimmune diabetes in adults (LADA). The aim of the current study was to compare a newly developed electrochemiluminescence (ECL)-GAD65 antibody assay with the established radiobinding assay, and to explore whether the new assay could be used to define LADA more precisely. METHODS: RESULTS: Eighty-eight out of 141 serum samples (62.4%) from LADA patients were GAD65 antibody-positive by ECL assay. Compared with ECL-GAD65 antibody-negative patients, ECL-GAD65 antibody-positive patients were leaner (P<0.0001), had poorer β-cell function (P<0.05), and were more likely to have other diabetes-associated autoantibodies. The β-cell function of ECL-GAD65 antibody-positive patients was similar to that of type 1 diabetes mellitus patients, whereas ECL-GAD65 antibody-negative patients were more similar to type 2 diabetes mellitus patients. CONCLUSION: Patients with ECL-GAD65 antibody-negative share a similar phenotype with type 2 diabetes mellitus patients, whereas patients with ECL-GAD65 antibody-positive resemble those with type 1 diabetes mellitus. Thus, the detection of GADA using ECL may help to identify the subtype of LADA.
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Authors | Yuxiao Zhu, Li Qian, Qing Liu, Jing Zou, Ying Zhou, Tao Yang, Gan Huang, Zhiguang Zhou, Yu Liu |
Journal | Diabetes & metabolism journal
(Diabetes Metab J)
Vol. 44
Issue 2
Pg. 260-266
(04 2020)
ISSN: 2233-6087 [Electronic] Korea (South) |
PMID | 31769235
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2020 Korean Diabetes Association. |
Chemical References |
- Autoantibodies
- C-Peptide
- Glutamate Decarboxylase
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Topics |
- Adolescent
- Adult
- Autoantibodies
(blood)
- C-Peptide
(blood)
- Case-Control Studies
- Diabetes Mellitus, Type 1
(blood, immunology)
- Diabetes Mellitus, Type 2
(blood, immunology)
- Female
- Glutamate Decarboxylase
(immunology)
- Humans
- Islets of Langerhans
(immunology, physiopathology)
- Latent Autoimmune Diabetes in Adults
(blood, immunology, metabolism)
- Male
- Middle Aged
- Phenotype
- Young Adult
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