Dynamics of tumor-specific cfDNA in response to therapy in multiple myeloma patients.

Abstract

OBJECTIVES:

Progress in multiple myeloma treatment allows patients to achieve deeper responses, for which the assessment of minimal residual disease (MRD) is critical. Typically, bone marrow samples are used for this purpose; however, this approach is site-limited. Liquid biopsy represents a minimally invasive and more comprehensive technique that is not site-limited, but equally challenging.

METHODS:

While majority of current data comes from short-term studies, we present a long-term study on blood-based MRD monitoring using tumor-specific cell-free DNA detection by ASO-qPCR. One hundred and twelve patients were enrolled into the study, but long-term sampling and analysis were feasible only in 45 patients.

RESULTS:

We found a significant correlation of quantity of tumor-specific cell-free DNA levels with clinically meaningful events [induction therapy (P = .004); ASCT (P = .012)]. Moreover, length of cfDNA fragments is associated with better treatment response of patients.

CONCLUSIONS:

These results support the concept of tumor-specific cell-free DNA as a prognostic marker.

• Authors: David Vrabel, Lenka Sedlarikova, Lenka Besse, Lucie Rihova, Renata Bezdekova, Martina Almasi, Veronika Kubaczkova, Lucie Brožová, Jiri Jarkovsky, Hana Plonkova, Tomas Jelinek, Viera Sandecka, Martin Stork, Ludek Pour, Sabina Sevcikova, Roman Hajek
• Journal: European journal of haematology (Eur J Haematol) Vol. 104 Issue 3 Pg. 190-197 (Mar 2020) ISSN: 1600-0609 [Electronic] England
• PMID: 31763708 (Publication Type: Journal Article)
• Copyright: © 2019 The Authors. European Journal of Haematology published by John Wiley & Sons Ltd.

Chemical References

• Biomarkers, Tumor
• Circulating Tumor DNA
• Immunoglobulin Heavy Chains

Topics

• Biomarkers, Tumor
• Circulating Tumor DNA
• Combined Modality Therapy
• Disease Management
• Flow Cytometry
• Humans
• Immunoglobulin Heavy Chains (genetics)
• Multiple Myeloma (diagnosis, genetics, therapy)
• Neoplasm, Residual (diagnosis, genetics)
• Outcome Assessment, Health Care
• Polymerase Chain Reaction
• Treatment Outcome