Solid Phase 11C-Methylation, Purification and Formulation for the Production of PET Tracers.

Abstract

Routine production of radiotracers used in positron emission tomography (PET) mostly relies on wet chemistry where the radioactive synthon reacts with a non-radioactive precursor in solution. This approach necessitates purification of the tracer by high performance liquid chromatography (HPLC) followed by reformulation in a biocompatible solvent for human administration. We recently developed a novel 11C-methylation approach for the highly efficient synthesis of carbon-11 labeled PET radiopharmaceuticals, taking advantage of solid phase cartridges as disposable "3-in-1" units for the synthesis, purification and reformulation of the tracers. This approach obviates the use of preparative HPLC and reduces the losses of the tracer in transfer lines and due to radioactive decay. Furthermore, the cartridge-based technique improves synthesis reliability, simplifies the automation process and facilitates compliance with the Good Manufacturing Practice (GMP) requirements. Here, we demonstrate this technique on the example of production of a PET tracer Pittsburgh compound B ([11C]PiB), a gold standard in vivo imaging agent for amyloid plaques in the human brains.

• Authors: Thomas A Singleton, Mehdi Boudjemeline, Robert Hopewell, Dean Jolly, Hussein Bdair, Alexey Kostikov
• Journal: Journal of visualized experiments : JoVE (J Vis Exp) Issue 152 (10 24 2019) ISSN: 1940-087X [Electronic] United States
• PMID: 31710043 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Video-Audio Media)

Chemical References

• Carbon Radioisotopes
• Carbon-11

Topics

• Carbon Radioisotopes (metabolism)
• Humans
• Methylation
• Positron-Emission Tomography (methods)
• Reproducibility of Results