Isorhamnetin exerts neuroprotective effects in STZ-induced diabetic rats via attenuation of oxidative stress, inflammation and apoptosis.

Abstract

OBJECTIVE:

Isorhamnetin, a derivative of quercetin, exerts antioxidant and anti-inflammatory effects in different diseases, and we examined its protective effects against diabetes-related changes in the brain.

METHODS:

A single dose of a freshly prepared solution of streptozotocin (STZ) (60 mg/kg body weight) was intraperitoneally injected to establish STZ-induced diabetic model in male Wistar rats. The animals were randomly divided into four groups: control, control + isorhamnetin, diabetic, diabetic + isorhamnetin. Isorhamnetin at a dose of 10 mg/kg body weight was intraperitoneally administrated once a day for 12 weeks. Formalin and tail immersion tests were performed to evaluate the severity of pain. Astrogliosis markers such as GFAP and APO-E4, DNA fragments, MDA level, and TNFα expressions were evaluated using ELISA assay. Neuronal density in the hippocampus region was evaluated using Nissl staining. The method of Ellman and fluorescent probe 2, 7-dichlorofluorescein diacetate (DCFH-DA) was used to measure brain acetyl-cholinesterase activity and detect reactive nitrogen and oxygen species (RNS and ROS), respectively.

RESULTS:

Isorhamnetin reduced pain, blood glucose levels, and increased body weight significantly compared to control. Moreover, isorhamnetin inhibited astroglial activation, acetyl-cholinesterase activity, oxidative stress, apoptosis, and inflammation.

CONCLUSION:

These findings suggested that isorhamnetin has potential effects as neuroprotective agents against diabetes-related changes in the brain.

• Authors: Nida Jamali-Raeufy, Tourandokht Baluchnejadmojarad, Mehrdad Roghani, Sara Keimasi, Mina Goudarzi
• Journal: Journal of chemical neuroanatomy (J Chem Neuroanat) Vol. 102 Pg. 101709 (12 2019) ISSN: 1873-6300 [Electronic] Netherlands
• PMID: 31698018 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2019 Elsevier B.V. All rights reserved.

Chemical References

• Blood Glucose
• Neuroprotective Agents
• Reactive Nitrogen Species
• Reactive Oxygen Species
• 3-methylquercetin
• Malondialdehyde
• Quercetin

Topics

• Animals
• Apoptosis (drug effects)
• Blood Glucose (metabolism)
• Body Weight (drug effects)
• DNA Fragmentation (drug effects)
• Diabetes Mellitus, Experimental (drug therapy, metabolism)
• Inflammation (drug therapy, metabolism)
• Male
• Malondialdehyde (metabolism)
• Neurons (drug effects, metabolism)
• Neuroprotective Agents (pharmacology, therapeutic use)
• Oxidative Stress (drug effects)
• Pain (drug therapy, metabolism)
• Pain Measurement
• Quercetin (analogs & derivatives, pharmacology, therapeutic use)
• Rats
• Rats, Wistar
• Reactive Nitrogen Species (metabolism)
• Reactive Oxygen Species (metabolism)