Diet supplementation with commercial enzymatically-hydrolyzed egg white peptides ameliorates the severity of allergy in a mouse model of egg white allergy.

Abstract

BACKGROUND:

Commercial spray-dried egg white (S-EW) is often used for oral immunotherapy (OIT). Peptifine® (PF), a commercial preparation of enzymatically-hydrolyzed egg white (EW) peptides, is considered safe OIT food.

OBJECTIVE:

Here, we examined the therapeutic effect of PF or S-EW in a mouse model of EW allergy.

METHODS:

Sensitized female BALB/c mice underwent 4-week OIT by supplementation of 19% casein diet with 1% PF (PF group) or S-EW (S-EW group), and non-supplementation of 20% casein diet (non-OIT group). Non-sensitized mice acted as the non-allergy group. Oral and intraperitoneal EW challenges were performed and allergic biomarkers were determined.

RESULTS:

Changes in rectal temperature after oral challenge were comparable in the allergy groups. However, after intraperitoneal challenge, rectal temperature decrease in the PF and S-EW groups was lower than in the non-OIT group. After 4 weeks, plasma levels of ovalbumin- and ovomucoid-specific antibodies were assessed; IgE levels in the PF and S-EW groups were lower than those in the non-OIT group. Interleukin-4 secretion after EW challenge was significantly lower in splenocyte cultures derived from PF and S-EW groups than in the non-OIT group. Furthermore, the percentage of CD4+ Foxp3+ splenocytes in the PF group was significantly higher than that in the non-OIT and S-EW groups. Oral gavage of 40 mg PF did not induce an allergic response in sensitized mice with EW.

CONCLUSIONS:

Diet supplementation with 1% PF mildly ameliorated the severity of allergy in mouse with EW allergy, indicating that PF is a safe OIT food.

• Authors: Akihiro Maeta, Risako Katahira, Matsushima Marin, Kyoko Takahashi
• Journal: Asian Pacific journal of allergy and immunology (Asian Pac J Allergy Immunol) Vol. 40 Issue 4 Pg. 359-367 (Dec 2022) ISSN: 0125-877X [Print] Thailand
• PMID: 31677613 (Publication Type: Journal Article)

Chemical References

• Caseins
• Immunoglobulin E
• Allergens
• Peptides

Topics

• Female
• Mice
• Animals
• Egg White
• Caseins
• Immunoglobulin E
• Desensitization, Immunologic
• Egg Hypersensitivity (therapy)
• Diet
• Allergens
• Peptides