Abstract | BACKGROUND: OBJECTIVES: Here we present a novel ImmunoCAPTM automated immunofluorescence research assay for measurement of EDN and its potential use in diagnosis of asthmatic children. METHODS: We report the analytical performance of the assay in serum, heparin- and EDTA-plasma in terms of precision, linearity, sensitivity, interfering substances and specimen handling. We also compared the EDN research assay with established methods for asthma diagnosis: fraction of exhaled nitric oxide (FeNO) and blood eosinophil fraction (EOS%) to demonstrate the diagnostic value of EDN in childhood asthma. RESULTS: The total precision (measured using percentage CV) was ≤5.8% for both serum and plasma. The dilution analysis yielded linear results across the dynamic range of the assay for both serum and plasma. No notable interferences of endogenous substances were observed.The median concentration of EDN was significantly higher in the asthma group compared to the healthy controls and the EDN correlates well with EOS%. CONCLUSIONS: we have shown that EDN can be measured reliably and robustly with the ImmunoCAP platform in both serum and plasma samples. EDN can be used to distinguish asthmatic from healthy children and correlates well with EOS% and could be a valuable complement to both EOS% and FeNO.
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Authors | Niclas Rydell, Mizuho Nagao, Helena Ekoff, Anders Sjölander, Magnus Borres, Takao Fujisawa |
Journal | Practical laboratory medicine
(Pract Lab Med)
Vol. 17
Pg. e00138
(Nov 2019)
ISSN: 2352-5517 [Print] Netherlands |
PMID | 31649990
(Publication Type: Journal Article)
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Copyright | © 2019 The Authors. |