Abstract | PURPOSE: MATERIALS AND METHODS: Urethral tissue was excised from patients with lichen sclerosus who were undergoing urethroplasty of urethral stricture disease at a single institution. A tissue microarray was created with cores from each sample. Immunohistochemistry was performed to compare protein expression related to inflammation, cell cycle disruption, oxidative stress, hormone receptor status and infection. Stricture recurrence was defined by the need for a subsequent unanticipated procedure for urethral stricture disease. RESULTS: CONCLUSIONS: To our knowledge this is the first study to use tissue protein expression to identify risk factors for urethroplasty failure among men with lichen sclerosus urethral stricture disease. Our findings suggest that recurrent lichen sclerosus strictures demonstrate a suppressed inflammatory response, a decreased cell turnover rate, and poor oxygenation and nutrient delivery. Prospective studies are needed to clarify the role of these pathways in the pathophysiology of lichen sclerosus urethral stricture disease, determine whether preoperative biopsy can predict urethroplasty success, help counsel patients and develop future treatments.
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Authors | Alison C Levy, Matthew Moynihan, Jennifer A Bennett, Travis Sullivan, Kristian Stensland, Brendan M Browne, Ariel Fredrick, Jaime A Cavallo, Elizabeth Pagura, Rafael Tua-Caraccia, Kimberly M Rieger-Christ, Alex J Vanni |
Journal | The Journal of urology
(J Urol)
Vol. 203
Issue 4
Pg. 773-778
(04 2020)
ISSN: 1527-3792 [Electronic] United States |
PMID | 31621469
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Biomarkers
(analysis, metabolism)
- Biopsy
- Female
- Follow-Up Studies
- Gene Expression Profiling
- Humans
- Immunohistochemistry
- Lichen Sclerosus et Atrophicus
(complications, pathology, surgery)
- Male
- Middle Aged
- Preoperative Period
- Prognosis
- Prospective Studies
- Plastic Surgery Procedures
(methods)
- Recurrence
- Reoperation
(statistics & numerical data)
- Tissue Array Analysis
- Treatment Outcome
- Urethra
(pathology, surgery)
- Urethral Stricture
(etiology, pathology, surgery)
- Urologic Surgical Procedures, Male
(methods)
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