Abstract | OBJECTIVE: METHODS: We compared the effects of CSF containing leucine-rich glioma inactivated 1 (LGI1), contactin-associated protein-like 2 (CASPR2), and γ- aminobutyric acid receptor B (GABAB R) antibodies on ex vivo electrophysiological parameters after stereotactic hippocampal inoculation into mice. Whole-cell patch-clamp and extracellular recordings from CA1 pyramidal neurons and CA3-CA1 field recordings in ex vivo murine brain slices were used to study neuronal function. RESULTS: By comparison to control CSF, AE CSFs increased the probability of glutamate release from CA3 neurons. In addition, LGI1- and CASPR2 antibodies containing CSFs induced epileptiform activity at a population level following Schaffer collateral stimulation. CASPR2 antibody containing CSF was also associated with higher spontaneous firing of CA1 pyramidal neurons. On the contrary, GABAB R antibody containing CSF did not elicit changes in intrinsic neuronal activity and field potentials. INTERPRETATION: Using patient CSF, we have demonstrated that the AE-associated antibodies against LGI1 and CASPR2 are able to increase hippocampal CA1 neuron excitability, facilitating epileptiform activity. These findings provide in vivo pathogenic insights into neuronal dysfunction in these conditions.
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Authors | Michele Romoli, Paraskevi Krashia, Arjune Sen, Diego Franciotta, Matteo Gastaldi, Annalisa Nobili, Andrea Mancini, Elena Nardi Cesarini, Pasquale Nigro, Nicola Tambasco, Nicola B Mercuri, Lucilla Parnetti, Massimiliano Di Filippo, Marcello D'Amelio, Sarosh R Irani, Cinzia Costa, Paolo Calabresi |
Journal | Annals of clinical and translational neurology
(Ann Clin Transl Neurol)
Vol. 6
Issue 11
Pg. 2261-2269
(11 2019)
ISSN: 2328-9503 [Electronic] United States |
PMID | 31617317
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | © 2019 The Authors. Annals of Clinical and Translational Neurology published by Wiley Periodicals, Inc on behalf of American Neurological Association. |
Chemical References |
- Autoantibodies
- Autoantigens
- CNTNAP2 protein, human
- Intracellular Signaling Peptides and Proteins
- LGI1 protein, human
- Membrane Proteins
- Nerve Tissue Proteins
- Receptors, GABA-B
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Topics |
- Animals
- Autoantibodies
(cerebrospinal fluid, pharmacology)
- Autoantigens
(immunology)
- Autoimmune Diseases of the Nervous System
(complications, immunology)
- Encephalitis
(complications, immunology)
- Epilepsy
(etiology, immunology)
- Hippocampus
(drug effects)
- Humans
- Intracellular Signaling Peptides and Proteins
(immunology)
- Male
- Membrane Proteins
(immunology)
- Mice
- Mice, Inbred C57BL
- Nerve Tissue Proteins
(immunology)
- Neurons
(drug effects)
- Receptors, GABA-B
(immunology)
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