Higher frequency of the CTLA-4+ LAG-3+ T-cell subset in patients with newly diagnosed acute myeloid leukemia.

Abstract

AIM:

Immune suppression based on alternative regulation of immune checkpoint proteins, for example, programmed cell death receptor-1 (PD-1) and cytotoxic T lymphocyte-associated molecule-4 (CTLA-4), which results in T-cell exhaustion, contributes to cancer development and progression. In this study, we sought to characterize the distribution of CTLA-4 and T-cell lymphocyte activation gene-3 (LAG-3) expression on exhausted T cells in different T-cell subsets from patients with acute myeloid leukemia (AML).

METHODS:

The coexpression of CTLA-4 and LAG-3 on exhausted CD244+ and CD57+ T cells from the CD3+ , CD4+ , and CD8+ T-cell subsets in peripheral blood from 12 patients with newly diagnosed AML was analyzed by multicolor flow cytometry assay.

RESULTS:

A significantly higher percentage of CTLA-4+ CD3+ , CD4+ and CD8+ T cells was found in patients with AML. In addition, higher numbers of both CTLA-4+ CD244+ and CTLA-4+ CD57+ CD3+ T cells were detected. Interestingly, the increased CTLA-4+ CD244+ T cells were predominantly CD4+ T cells. In contrast, the increased CTLA-4+ CD57+ T cells primarily consisted of the CD8+ T-cell subset. A high proportion of LAG-3+ T cells was found in only a few cases with AML; however, a significantly higher proportion of coexpression of CTLA-4 and LAG-3 in the CD3+ and CD8+ T-cell subsets was detected.

CONCLUSION:

We for the first time observed higher CTLA-4+ CD244+ CD4+ , CTLA-4+ CD57+ CD8+ , CTLA-4+ LAG-3+ CD3+ and CTLA-4+ LAG-3+ CD8+ T cells in patients with AML, whereas the upregulated expression of LAG-3 on T cells was only found in a subset of the cases. These data may provide further information by complementing the heterogeneity of immune checkpoints expression in AML.

• Authors: Youchun Chen, Jiaxiong Tan, Shuxin Huang, Xin Huang, Jingying Huang, Jie Chen, Zhi Yu, Yuhong Lu, Jianyu Weng, Xin Du, Yangqiu Li, Xianfeng Zha, Shaohua Chen
• Journal: Asia-Pacific journal of clinical oncology (Asia Pac J Clin Oncol) Vol. 16 Issue 2 Pg. e12-e18 (Apr 2020) ISSN: 1743-7563 [Electronic] Australia
• PMID: 31612643 (Publication Type: Journal Article)
• Copyright: © 2019 John Wiley & Sons Australia, Ltd.

Chemical References

• CTLA-4 Antigen

Topics

• Adult
• Aged
• Aged, 80 and over
• CTLA-4 Antigen (metabolism)
• Female
• Humans
• Leukemia, Myeloid, Acute (genetics, pathology)
• Male
• Middle Aged
• Prognosis
• T-Lymphocytes, Cytotoxic (metabolism)