A fluorescent lateral flow biosensor for the quantitative detection of Vaspin using upconverting nanoparticles.

Abstract

Vaspin is a protein present in human serum that can cause type-2 diabetes, obesity, and other cardiovascular diseases. We report fluorescent upconverting nanoparticles (UCNPs)-based lateral flow biosensor for ultrasensitive detection of Vaspin. A pair (primary and secondary) of cognate aptamers was used that has duo binding with Vaspin. UCNPs with a diameter of around 100 nm were used as a tag to label a detection probe (secondary aptamer). A primary aptamer (capture probe) was immobilized on the test zone. Sandwich type hybridization reactions among the conjugate probe, target Vaspin, and primary aptamer were performed on the lateral flow biosensor. In the presence of target Vaspin, UCNPs were captured on the test zone of the biosensor and the fluorescent intensity of the captured UCNPs was measured through a colorimetric app under NIR. Fluorescence intensity indicates the quantity of Vaspin present in the sample. A range of Vaspin concentration across 0.1-55 ng ml-1 with a Limit of detection (LOD) 39 pg ml-1 was tested through this UCNPs based LFSA with high sensitivity, reproducibility and repeatability, whereas it's actual range in human blood is from 0.1 to 7 ng ml-1. Therefore, this research provides a well-suited lateral flow strip with an ultrasensitive and low-cost approach for the early diagnosis of type-2 diabetes and this could be applied to any targets with a duo of aptamers generated.

• Authors: Muhsin Ali, Memoon Sajid, Muhammad Asad Ullah Khalid, Soo Wan Kim, Jong Hwan Lim, Dongeun Huh, Kyung Hyun Choi
• Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (Spectrochim Acta A Mol Biomol Spectrosc) Vol. 226 Pg. 117610 (Feb 05 2020) ISSN: 1873-3557 [Electronic] England
• PMID: 31606675 (Publication Type: Evaluation Study, Journal Article)
• Copyright: Copyright © 2019. Published by Elsevier B.V.

Chemical References

• Aptamers, Nucleotide
• Fluorescent Dyes
• SERPINA12 protein, human
• Serpins

Topics

• Aptamers, Nucleotide (analysis)
• Biosensing Techniques (methods)
• Blood Chemical Analysis (methods)
• Diabetes Mellitus, Type 2 (blood, diagnosis)
• Early Diagnosis
• Fluorescence
• Fluorescent Dyes (chemical synthesis, chemistry, pharmacokinetics)
• Humans
• Limit of Detection
• Nanoparticles (chemistry)
• Protein Binding
• Reproducibility of Results
• Serpins (analysis, metabolism)