Abstract | BACKGROUND:
Epidermal growth factor receptor (EGFR) gene mutation status is essential to the optimal management of lung adenocarcinoma. Liquid biopsy has advantages such as noninvasiveness, speediness, and convenience. This study aimed to detect EGFR gene mutations using next-generation sequencing (NGS) from different types of body fluids from patients with lung adenocarcinoma. METHODS: RESULTS: The EGFR gene mutation abundance of body fluid supernatant free DNA was higher than that of body fluid sedimentary tumor cells and plasma free DNA specimens (100% vs. 90% vs. 80%, respectively, all P < 0.05). The results of EGFR mutation from the body fluid supernatants were consistent with the results from the tissue biopsy. CONCLUSIONS: This study showed that compared with body fluid sediment tumor cells and plasma free DNA samples, body fluid supernatant free DNA has a higher detection rate and sensitivity of tumor-specific mutations. Free DNA obtained from body fluid supernatants could be used as high-quality specimens for gene mutation detection in patients with lung cancer. This could be applied in treatment decisions and patient management.
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Authors | Ping Zhang, Xiaonan Wu, Min Tang, Xin Nie, Lin Li |
Journal | Thoracic cancer
(Thorac Cancer)
Vol. 10
Issue 12
Pg. 2218-2224
(12 2019)
ISSN: 1759-7714 [Electronic] Singapore |
PMID | 31602787
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2019 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd. |
Chemical References |
- Biomarkers, Tumor
- Cell-Free Nucleic Acids
- EGFR protein, human
- ErbB Receptors
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Topics |
- Adenocarcinoma of Lung
(genetics, pathology)
- Alleles
- Biomarkers, Tumor
- Body Fluids
- Cell-Free Nucleic Acids
- DNA Mutational Analysis
- ErbB Receptors
(genetics)
- Female
- Genotype
- High-Throughput Nucleotide Sequencing
- Humans
- Male
- Mutation
- Pleural Effusion, Malignant
(pathology)
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