The role of flavonoid antioxidant, morin in improving procarbazine-induced oxidative stress on testicular function in rat.

Abstract

BACKGROUND:

Procarbazine (PCZ) is an effective chemotherapeutic drug used in the treatment of lymphoma; however, oxidative stress-mediated testicular toxicity is a major side effect. Recently, therapeutic intervention using flavonoids against oxidative stress-related pathologies is gaining more attention. Morin (MOR) is a natural flavonoid with proven antioxidant activity. This study was designed therefore to evaluate the potential role of MOR in ameliorating PCZ-induced testicular oxidative stress and altered sperm quality in rat model.

METHODS:

A total of 24 male Wistar rats (170-180 g) were randomly assigned into 4 treatment groups: I, control; II, PCZ (2 mg/kg b.w.); III, PCZ (2 mg/kg b.w.) + MOR (100 mg/kg b.w.) simultaneously administered and IV, MOR (100 mg/kg b.w.), and all treatments lasted 14 days.

RESULTS:

PCZ treatment displayed significant reduction in sperm number, sperm motility, percentage normal sperm cells, and daily sperm production rate. Meanwhile the activities of testicular enzymes: gamma-glutamyl transferase, acid phosphatase, and lactate dehydrogenase were significantly altered in the PCZ group compared to control. Furthermore, PCZ caused a significant reduction in levels of glutathione and ascorbic acid as well as activities superoxide dismutase, catalase, glutathione peroxidase, and glutathione S-transferase in the testes of PCZ-treated rats. A significant increase in testicular malondialdehyde level was also observed in the PCZ group. MOR treatment, however, significantly restored the altered sperm parameters and biochemical markers in the testis.

CONCLUSIONS:

Our data suggest that MOR administration protected against PCZ-induced testicular and spermatotoxicity in rat, by improving testicular antioxidant system.

• Authors: Ebenezer Tunde Olayinka, Ayokanmi Ore, Oluwatobi Adewumi Adeyemo, Olaniyi Solomon Ola
• Journal: Porto biomedical journal (Porto Biomed J) 2019 Jan-Feb Vol. 4 Issue 1 Pg. e28 ISSN: 2444-8672 [Electronic] United States
• PMID: 31595255 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 The Authors. Published by Wolters Kluwer Health, Inc. on behalf of PBJ-Associação Porto Biomedical/Porto Biomedical Society. All rights reserved.