Abstract | PURPOSE: METHODS: Ten resistance-trained men were randomized in a crossover, double-blind, placebo (PLA)-controlled manner to ingest either a noncaloric PLA or 3 g/kg of CHO beverage throughout recovery from resistance exercise. Muscle biopsies were collected at rest, immediately after a single bout of intense lower body resistance exercise, and after 3 hr of recovery. RESULTS: CHO ingestion elevated plasma glucose and insulin concentrations throughout recovery compared with PLA ingestion. The ERK pathway (phosphorylation of ERK1/2 [Thr202/Tyr204], RSK [Ser380], and p70S6K [Thr421/Ser424]) was markedly activated immediately after resistance exercise, without any effect of CHO supplementation. The phosphorylation state of AKT (Thr308) was unchanged postexercise in the PLA trial and increased at 3 hr of recovery above resting with ingestion of CHO compared with PLA. Despite stimulating-marked phosphorylation of AKT, CHO ingestion did not enhance resistance exercise-induced phosphorylation of p70S6K (Thr389) and rpS6 (Ser235/236 and Ser240/244). CONCLUSION: CHO supplementation after resistance exercise and hyperinsulinemia does not influence the ERK pathway nor the mTORC1 target p70S6K and its downstream proteins, despite the increased AKT phosphorylation.
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Authors | Vandre C Figueiredo, Michelle M Farnfield, Megan L R Ross, Petra Gran, Shona L Halson, Jonathan M Peake, David Cameron-Smith, James F Markworth |
Journal | International journal of sport nutrition and exercise metabolism
(Int J Sport Nutr Exerc Metab)
Vol. 29
Issue 6
Pg. 664-670
(Nov 01 2019)
ISSN: 1543-2742 [Electronic] United States |
PMID | 31592623
(Publication Type: Journal Article, Randomized Controlled Trial)
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Chemical References |
- Blood Glucose
- Dietary Carbohydrates
- Insulin
- Mechanistic Target of Rapamycin Complex 1
- Proto-Oncogene Proteins c-akt
- Extracellular Signal-Regulated MAP Kinases
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Topics |
- Blood Glucose
(metabolism)
- Cross-Over Studies
- Dietary Carbohydrates
(pharmacology)
- Double-Blind Method
- Extracellular Signal-Regulated MAP Kinases
(metabolism)
- Humans
- Insulin
(blood)
- Male
- Mechanistic Target of Rapamycin Complex 1
(metabolism)
- Proto-Oncogene Proteins c-akt
(metabolism)
- Resistance Training
- Young Adult
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