Abstract | PURPOSE: METHODS: Amoebicidal and cysticidal assays were performed against both trophozoites and cysts of Acanthamoeba castellanii (ATCC 50370) and Acanthamoeba polyphaga (ATCC 30461). Test agents included topical ophthalmic preparations of common anesthetics, antivirals, antibiotics, and biocides. Organisms were exposed to serial two-fold dilutions of the test compounds in the wells of a microtiter plate to examine the effect on Acanthamoeba spp. In addition, the toxicity of each of the test compounds was determined against a mammalian cell line. RESULTS: CONCLUSIONS: TRANSLATIONAL RELEVANCE: Ophthalmologists should be aware that certain topical anesthetics and ophthalmic preparations containing BAC prior to specimen sampling may affect the viability of Acanthamoeba spp. in vivo, resulting in false-negative results in diagnostic tests.
|
Authors | Wayne Heaselgrave, Anas Hamad, Steven Coles, Scott Hau |
Journal | Translational vision science & technology
(Transl Vis Sci Technol)
Vol. 8
Issue 5
Pg. 17
(Sep 2019)
ISSN: 2164-2591 [Print] United States |
PMID | 31588380
(Publication Type: Journal Article)
|
Copyright | Copyright 2019 The Authors. |