Caspase recruitment domain family member 10 regulates carbamoyl phosphate synthase 1 and promotes cancer growth in bladder cancer cells.

Abstract

Bladder cancer, which can be divided into non-muscle-invasive and muscle-invasive bladder cancer, is the most common urinary cancer in the United States. Caspase recruitment domain family member 10 (CARD10), also named CARD-containing MAGUK protein 3 (CARMA3), is a member of the CARMA family and may activate the nuclear factor kappa B (NF-κB) pathway. We utilized RNA sequencing and metabolic mass spectrometry to identify the molecular and metabolic feature of CARD10. The signalling pathway of CARD10 was verified by Western blotting analysis and functional assays. RNA sequencing and metabolic mass spectrometry of CARD10 knockdown identified the metabolic enzyme carbamoyl phosphate synthase 1 (CPS1) in the urea cycle as the downstream gene regulated by CARD10. We confirmed that CARD10 affected cell proliferation and nucleotide metabolism through regulating CPS1. We indicated that CARD10 promote bladder cancer growth via CPS1 and maybe a potential therapeutic target in bladder cancer.

• Authors: Xi Liu, Xiaotong Zhang, Jianbin Bi, Zhenhua Li, Zhe Zhang, Chuize Kong
• Journal: Journal of cellular and molecular medicine (J Cell Mol Med) Vol. 23 Issue 12 Pg. 8128-8138 (12 2019) ISSN: 1582-4934 [Electronic] England
• PMID: 31565867 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2019 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

Chemical References

• CARD Signaling Adaptor Proteins
• CARD10 protein, human
• NF-kappa B
• Nucleotides
• CPS1 protein, human
• Carbamoyl-Phosphate Synthase (Ammonia)

Topics

• Animals
• CARD Signaling Adaptor Proteins (genetics, metabolism)
• Carbamoyl-Phosphate Synthase (Ammonia) (genetics, metabolism)
• Cell Line
• Cell Line, Tumor
• Cell Proliferation (genetics)
• Gene Expression Regulation, Neoplastic
• Humans
• Mass Spectrometry (methods)
• Mice, Inbred BALB C
• Mice, Nude
• NF-kappa B (metabolism)
• Nucleotides (metabolism)
• RNA Interference
• RNAi Therapeutics (methods)
• Sequence Analysis, RNA (methods)
• Signal Transduction (genetics)
• Urinary Bladder Neoplasms (genetics, metabolism, therapy)
• Xenograft Model Antitumor Assays (methods)