The specific features of free radical oxidation in patients with chronic obstructive pulmonary disease and arterial hypertension.

Abstract

The pathogenesis of both chronic obstructive pulmonary disease (COPD) and arterial hypertension (AH) is closely related to oxidative stress, which is characterized by an imbalance between the production of reactive oxygen species (ROS) and the antioxidant defense.

AIM:

The aim of the study was to assess the parameters of free-radical oxidation and to establish their correlation to spirogram findings in patients with COPD without comorbidity and in patients with COPD and AH.

MATERIALS AND METHODS:

Detection of intracellular ROS levels was performed by EPICS XL cytometer (Beckman Coulter, USA) with DCFH-DA and DHE. Serum levels of 8-isoprostane were assayed with ELISA, Cayman Chemicals (USA).

RESULTS:

Intracellular ROS levels suggest a probable increase in production of O2•- in patients with COPD and in patients with COPD+AH vs control. The direction of changes in production of H2O2 in the assessed patients was also identical. Serum levels of 8-isoprostane were found to be significantly increased in patients with COPD and in patients with COPD+AH. High levels of H2O2, O2•- and 8-isoprostane were significantly associated with low values of spirometry parameters in patients of both test groups, which suggested bronchial obstruction. In this respect, oxidative stress parameters were significantly negatively correlated with the values characteristic of impaired patency of largeand medium-sized bronchi.

CONCLUSIONS:

Oxidative stress is playing an important role in the mechanisms of COPD/AH comorbidity, since intracellular ROS levels were found to increase, leading to destruction of cell membrane. In the meantime, the degree of oxidative stress correlates with the severity of bronchial obstruction.

• Authors: Mariya Marushchak, Khrystyna Maksiv, Inna Krynytska
• Journal: Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego (Pol Merkur Lekarski) Vol. 47 Issue 279 Pg. 95-98 (Sep 25 2019) ISSN: 1426-9686 [Print] Poland
• PMID: 31557137 (Publication Type: Journal Article)
• Copyright: © 2019 MEDPRESS.

Chemical References

• Hydrogen Peroxide

Topics

• Humans
• Hydrogen Peroxide
• Hypertension (complications, physiopathology)
• Oxidation-Reduction
• Oxidative Stress
• Pulmonary Disease, Chronic Obstructive (complications, physiopathology)