The essential role of the transporter ABCG2 in the pathophysiology of erythropoietic protoporphyria.

Abstract

Erythropoietic protoporphyria (EPP) is an inherited disease caused by loss-of-function mutations of ferrochelatase, an enzyme in the heme biosynthesis pathway that converts protoporphyrin IX (PPIX) into heme. PPIX accumulation in patients with EPP leads to phototoxicity and hepatotoxicity, and there is no cure. Here, we demonstrated that the PPIX efflux transporter ABCG2 (also called BCRP) determines EPP-associated phototoxicity and hepatotoxicity. We found that ABCG2 deficiency decreases PPIX distribution to the skin and therefore prevents EPP-associated phototoxicity. We also found that ABCG2 deficiency protects against EPP-associated hepatotoxicity by modulating PPIX distribution, metabolism, and excretion. In summary, our work has uncovered an essential role of ABCG2 in the pathophysiology of EPP, which suggests the potential for novel strategies in the development of therapy for EPP.

• Authors: Pengcheng Wang, Madhav Sachar, Jie Lu, Amina I Shehu, Junjie Zhu, Jing Chen, Ke Liu, Karl E Anderson, Wen Xie, Frank J Gonzalez, Curtis D Klaassen, Xiaochao Ma
• Journal: Science advances (Sci Adv) Vol. 5 Issue 9 Pg. eaaw6127 (09 2019) ISSN: 2375-2548 [Electronic] United States
• PMID: 31555729 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• ATP Binding Cassette Transporter, Subfamily G, Member 2
• Abcg2 protein, mouse
• Protoporphyrins
• protoporphyrin IX
• Ferrochelatase

Topics

• ATP Binding Cassette Transporter, Subfamily G, Member 2 (genetics, metabolism)
• Animals
• Ferrochelatase (genetics, metabolism)
• Liver (metabolism, pathology)
• Mice
• Mice, Mutant Strains
• Protoporphyria, Erythropoietic (genetics, metabolism, pathology, physiopathology)
• Protoporphyrins (genetics, metabolism)
• Skin (metabolism, pathology)