A potent broadly neutralizing human RSV antibody targets conserved site IV of the fusion glycoprotein.

Abstract

Respiratory syncytial virus (RSV) infection is the leading cause of hospitalization and infant mortality under six months of age worldwide; therefore, the prevention of RSV infection in all infants represents a significant unmet medical need. Here we report the isolation of a potent and broadly neutralizing RSV monoclonal antibody derived from a human memory B-cell. This antibody, RB1, is equipotent on RSV A and B subtypes, potently neutralizes a diverse panel of clinical isolates in vitro and demonstrates in vivo protection. It binds to a highly conserved epitope in antigenic site IV of the RSV fusion glycoprotein. RB1 is the parental antibody to MK-1654 which is currently in clinical development for the prevention of RSV infection in infants.

• Authors: Aimin Tang, Zhifeng Chen, Kara S Cox, Hua-Poo Su, Cheryl Callahan, Arthur Fridman, Lan Zhang, Sangita B Patel, Pedro J Cejas, Ryan Swoyer, Sinoeun Touch, Michael P Citron, Dhanasekaran Govindarajan, Bin Luo, Michael Eddins, John C Reid, Stephen M Soisson, Jennifer Galli, Dai Wang, Zhiyun Wen, Gwendolyn J Heidecker, Danilo R Casimiro, Daniel J DiStefano, Kalpit A Vora
• Journal: Nature communications (Nat Commun) Vol. 10 Issue 1 Pg. 4153 (09 12 2019) ISSN: 2041-1723 [Electronic] England
• PMID: 31515478 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Antibodies, Viral
• Broadly Neutralizing Antibodies
• Epitopes
• Glycoproteins
• Viral Fusion Proteins

Topics

• Animals
• Antibodies, Monoclonal (isolation & purification)
• Antibodies, Viral (immunology)
• B-Lymphocytes (immunology)
• Binding Sites
• Broadly Neutralizing Antibodies (immunology)
• Conserved Sequence
• Disease Models, Animal
• Epitopes (immunology)
• Female
• Glycoproteins (immunology)
• Humans
• Immunologic Memory
• Models, Molecular
• Protein Binding
• Respiratory Syncytial Virus, Human (immunology)
• Sigmodontinae
• Viral Fusion Proteins (immunology)