Abstract |
MDG-1, a β-D- fructan polysaccharide extracted from the roots of Ophiopogon japonicus, had preventive effect against obesity and hyperlipidemia in high-fat diet (HFD)-induced obesity mice. Interestingly, MDG-1, as an inulin-type fructan, is poorly absorbed and its possible mechanism against lipid disturbance remained unclear. The present study aimed to investigate the benefits of MDG-1 treatment on NAFLD model and elucidate mechanism from the perspective of gut-liver axis, especially about gut microbiota, short chain fatty acids (SCFAs) and hepatic lipid metabolism. In this study, after two months HFD feeding, C57BL/6J male mice were randomly divided into HFD group and various MDG-1 dose group. Results showed that MDG-1 markedly blocked weight gain, and ameliorated lipid accumulation, liver damage and macrovesicular steatosis. MDG-1 could restore gut microbiota balance and increase relative abundance of beneficial bacteria, especially SCFAs-producing bacteria. After degradation and utilization by the gut microbiota, MDG-1 could increase the contents of acetic acid and valeric acid, thus regulating inflammatory responses and hepatic lipid metabolism. Specifically, MDG-1 enhanced expression of hepatic phosphorylation of adenosine monophosphate-activated protein kinase, accompanying by regulating hepatic adipogenesis and adipocyte differentiation, thereby inhibiting progress of NAFLD. Our findings may provide new ways in the treatment of hyperlipidemia and lipid-related metabolic syndrome.
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Authors | Xu Wang, Linlin Shi, Xinping Wang, Yi Feng, Yuan Wang |
Journal | International journal of biological macromolecules
(Int J Biol Macromol)
Vol. 141
Pg. 1013-1021
(Dec 01 2019)
ISSN: 1879-0003 [Electronic] Netherlands |
PMID | 31491513
(Publication Type: Journal Article)
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Copyright | Copyright © 2019 Elsevier B.V. All rights reserved. |
Chemical References |
- Fatty Acids, Volatile
- Polysaccharides
- Sterol Regulatory Element Binding Protein 1
- AMP-Activated Protein Kinases
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Topics |
- AMP-Activated Protein Kinases
(metabolism)
- Animals
- Body Weight
(drug effects)
- Energy Metabolism
(drug effects)
- Fatty Acids, Volatile
(blood, metabolism)
- Intestinal Mucosa
(drug effects, metabolism, pathology)
- Intestines
(drug effects)
- Liver
(drug effects, metabolism, pathology)
- Male
- Mice
- Mice, Inbred C57BL
- Non-alcoholic Fatty Liver Disease
(blood, drug therapy, metabolism, pathology)
- Ophiopogon
(chemistry)
- Polysaccharides
(pharmacology, therapeutic use)
- Signal Transduction
(drug effects)
- Sterol Regulatory Element Binding Protein 1
(metabolism)
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