Water extract of Cnidii Rhizoma suppresses RANKL-induced osteoclastogenesis in RAW 264.7 cell by inhibiting NFATc1/c-Fos signaling and prevents ovariectomized bone loss in SD-rat.

Abstract

BACKGROUND:

Cnidii Rhizoma is the dried root stem of Cnidium officinale Makino. Cnidii Rhizoma (CR) has been used to treat menstrual irregularity, menstrual pain, and menopause in Korea. However, the effects and mechanisms of CR on RANKL-induced osteoclastogenesis pathway remain to be elucidated. In this study, we investigated the effects of CR on the inhibition of bone resorption of osteoclast and its mechanism RANK signaling pathway.

METHODS:

The anti-osteoclastogenesis of water extract of CR was measured using RAW 264.7 cell. Tartrate-resistant acid phosphatase (TRAP) assay, pit assay, reverse transcription polymerase chain reaction (RT-PCR) and western blot were performed. Moreover, the effects of CR were determined with an in vivo model using ovariectomized (OVX) rats.

RESULTS:

CR extract suppressed osteoclastogenesis, its activity and bone resorption activity through decreasing gene of osteoclast-related such as nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1), c-Fos, etc. Moreover, CR extract prevented the bone loss in OVX rats.

CONCLUSION:

These results show that CR has a positive effect on menopausal osteoporosis by suppressing osteoclastogenesis.

• Authors: Ka-Yeon Lee, Jae-Hyun Kim, Eun-Young Kim, Mijung Yeom, Hyuk-Sang Jung, Youngjoo Sohn
• Journal: BMC complementary and alternative medicine (BMC Complement Altern Med) Vol. 19 Issue 1 Pg. 207 (Aug 09 2019) ISSN: 1472-6882 [Electronic] England
• PMID: 31399090 (Publication Type: Journal Article)

Chemical References

• NFATC Transcription Factors
• Nfatc1 protein, mouse
• Plant Extracts
• Proto-Oncogene Proteins c-fos
• RANK Ligand
• Tnfsf11 protein, mouse

Topics

• Animals
• Bone Diseases, Metabolic (genetics, metabolism, physiopathology, prevention & control)
• Cnidium (chemistry)
• Female
• Humans
• Mice
• NFATC Transcription Factors (genetics, metabolism)
• Osteoclasts (drug effects, metabolism)
• Osteogenesis (drug effects)
• Ovariectomy
• Plant Extracts (administration & dosage)
• Proto-Oncogene Proteins c-fos (genetics, metabolism)
• RANK Ligand (genetics, metabolism)
• RAW 264.7 Cells
• Rats
• Republic of Korea
• Rhizome (chemistry)
• Signal Transduction (drug effects)