Temperature-responsive mixed-mode column containing temperature-responsive polymer-modified beads and anionic polymer-modified beads.

Abstract

We developed temperature-responsive mixed-mode columns packed with poly(N-isopropylacrylamide) (PNIPAAm)-modified beads and poly(2-acrylamido-2-methylpropane sulfonic acid) (PAMPS)-modified beads in various ratios. The PNIPAAm-modified silica beads and PAMPS-modified silica beads were prepared by surface-initiated atom transfer radical polymerization of N-isopropylacrylamide and 2-acrylamido-2-methylpropane sulfonic acid, respectively. We confirmed polymer modification of the silica beads by CHN elemental analysis, FTIR, zeta-potential measurements, and SEM. To determine the column separation efficiency, we examined the elution behaviors of cold medicine active ingredients and monoamines from each column. Analyte separation occurred on columns with PNIPAAm to PAMPS ratios of 1:20 and 1:10, whereas a column containing only PNIPAAm-modified beads did not retain the analytes. The analytes were retained on the columns through hydrophobic and electrostatic interactions with PNIPAAm and PAMPS, respectively. The separation performance improved with increasing column temperature because of dehydration of PNIPAAm and enhancement of hydrophobic interactions at elevated temperatures. The mixed-mode columns will be useful for separating basic bioactive compounds because the retention of analytes can be modulated by changing the column temperature and the composition.

• Authors: Kenichi Nagase, Maria Watanabe, Fumihiko Zen, Hideko Kanazawa
• Journal: Analytica chimica acta (Anal Chim Acta) Vol. 1079 Pg. 220-229 (Nov 04 2019) ISSN: 1873-4324 [Electronic] Netherlands
• PMID: 31387715 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 Elsevier B.V. All rights reserved.

Chemical References

• Acrylic Resins
• Alkaloids
• Amines
• Anion Exchange Resins
• Heterocyclic Compounds, Fused-Ring
• Polymers
• Sulfonic Acids
• poly-N-isopropylacrylamide
• poly(2-acrylamido-2-methyl-1-propanesulfonic acid)
• Silicon Dioxide

Topics

• Acrylic Resins (chemistry)
• Alkaloids (analysis)
• Amines (analysis)
• Anion Exchange Resins (chemistry)
• Chromatography, Ion Exchange (instrumentation, methods)
• Heterocyclic Compounds, Fused-Ring (analysis)
• Polymers (chemistry)
• Silicon Dioxide (chemistry)
• Sulfonic Acids (chemistry)
• Temperature