Effects of Docosahexaenoic Acid on Chemokine Expression in Human Conjunctival Fibroblasts.
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| Abstract |
Purpose: We assessed the production of chemokines by human conjunctival fibroblasts in response to inflammation and the effects of omega (ω)-3 fatty acids on chemokine expression.Methods: Primary cultures of human conjunctival fibroblasts were incubated with interleukin-4 (IL-4) and tumor necrosis factor-alpha (TNF-α). The expression of eotaxin-1 and RANTES in response to pretreatment with docosahexaenoic acid (DHA) was investigated. Moreover, western blotting was used to evaluate the effects of DHA on the activation of nuclear factor (NF)-κB and signal transducer and activator of transcription 6 (STAT6).Results: The expression of eotaxin-1 mRNA was significantly suppressed by pretreatment with DHA with IL-4 and TNF-α costimulation. RANTES expression was similarly suppressed, but the difference was not significant. The secretion of eotaxin-1 and RANTES was significantly lower in DHA-pretreated cells than in vehicle-treated cells. Western blotting for NF-κB and STAT6 showed that these proteins were downregulated in the DHA pretreatment group compared with those in the vehicle control group.Conclusion: The results of this study suggested that DHA could have applications in the management of allergic inflammation.
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| Authors | Hiroyuki Yazu, Kazumi Fukagawa, Naoko Okada, Hiroshi Fujishima |
| Journal | Current eye research (Curr Eye Res) Vol. 45 Issue 1 Pg. 81-86 (01 2020) ISSN: 1460-2202 [Electronic] England |
| PMID | 31364439 (Publication Type: Journal Article) |
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