Progenitor Cells Derived from Drain Waste Product of Open-Heart Surgery in Children.

Abstract

Human cardiac progenitor cells isolated from the same host may have advantages over other sources of stem cells. The aim of this study is to establish a new source of human progenitor cells collected from a waste product, pericardiac effusion fluid, after open-heart surgery in children with congenital heart diseases. The fluid was collected every 24 h for 2 days after surgery in 37 children. Mononuclear cells were isolated and expanded in vitro. These pericardial effusion-derived progenitor cells (PEPCs) exhibiting cardiogenic lineage markers, were highly proliferative and enhanced angiogenesis in vitro. Three weeks after stem cell transplantation into the ischemic heart in mice, cardiac ejection fraction was improved significantly without detectable progenitor cells. Gene expression profiles of the repaired hearts revealed activation of several known repair mechanisms including paracrine effects, cell migration, and angiogenesis. These progenitor cells may have the potential for heart regeneration.

• Authors: Tak-Wah Wong, Chung-Dann Kan, Wen-Tai Chiu, Kin Lam Fok, Ye Chun Ruan, Xiaohua Jiang, Junjiang Chen, Chiu-Ching Kao, I-Yu Chen, Hui-Chun Lin, Chia-Hsuan Chou, Chou-Wen Lin, Chun-Keung Yu, Stephanie Tsao, Yi-Ping Lee, Hsiao Chang Chan, Jieh-Neng Wang
• Journal: Journal of clinical medicine (J Clin Med) Vol. 8 Issue 7 (Jul 12 2019) ISSN: 2077-0383 [Print] Switzerland
• PMID: 31336927 (Publication Type: Journal Article)