The synthetic cannabinoid JWH-018 modulates Saccharomyces cerevisiae energetic metabolism.
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| Abstract |
Synthetic cannabinoids are a group of novel psychoactive substances with similar properties to Δ9-THC. Among the vast number of synthetic cannabinoids, designed to be tested in clinical trials, JWH-018 was the first novel psychoactive substance found in the recreational drug marketplace. The consumption of JWH-018 shows typical effects of CB1 agonists including sedation, cognitive dysfunction, tachycardia, postural hypotension, dry mouth, ataxia and psychotropic effects, but appeared to be more potent than Δ9-THC. However, studies on human cells have shown that JWH-018 toxicity depends on the cellular line used. Despite these studies, the underlying molecular mechanisms to JWH-018 action has not been clarified yet. To understand the impact of JWH-018 at molecular and cellular level, we used Saccharomyces cerevisiae as a model. The results showed an increase in yeast growth rate in the presence of this synthetic cannabinoid due to an enhancement in the glycolytic flux at expense of a decrease in pentose phosphate pathway, judging by 2D-Gel proteomic analysis, qRT-PCR experiments and ATP measurements. Overall, our results provide insights into molecular mechanisms of JWH-018 action, also indicating that Saccharomyces cerevisiae is a good model to study synthetic cannabinoids.
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| Authors | Carla Ferreira, Joana Couceiro, Carlos Família, Carolina Jardim, Pedro Antas, Cláudia N Santos, Tiago F Outeiro, Sandra Tenreiro, Alexandre Quintas |
| Journal | FEMS yeast research (FEMS Yeast Res) Vol. 19 Issue 5 (08 01 2019) ISSN: 1567-1364 [Electronic] England |
| PMID | 31329229 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Copyright | © FEMS 2019. |
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