Abstract | OBJECTIVES: Chronic rhinosinusitis is a common, costly condition often treated with endoscopic sinus surgery and intraoperative placement of intranasal sinus implant materials. Whereas these materials aid in postoperative healing, they also support bacterial biofilm formation and thus contribute to negative outcomes. This study examined pretreatment of sinus implant materials with antibody against an essential bacterial biofilm structural component, the DNABII family of DNA-binding proteins, as a strategy to prevent biofilm formation. METHODS: RESULTS: NTHI readily formed biofilms on all three materials in vitro. However, pretreatment of each material with IgG-enriched anti-IHF resulted in a significant decrease in bacterial burden compared to controls (P ≤ 0.05). Moreover, a significant and synergistic outcome was achieved with a cocktail of anti-IHF plus amoxicillin- clavulanate (P ≤ 0.05) with complete inhibition of NTHI biofilm formation on all three materials. CONCLUSIONS: Biofilm formation was well supported in vitro on three sinus implant materials that vary in composition and resorption characteristics; however, pretreatment of each with DNABII protein targeted antibodies in combination with a previously ineffective antibiotic was highly effective to prevent the formation NTHI biofilms. These data demonstrate the potential for clinical utility of pretreatment of sinus implant and additional surgical materials with anti-DNABII antibodies. LEVEL OF EVIDENCE:
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Authors | Lauren Martyn, Rishabh Sethia, Rachel Chon, Laura Novotny, Steven D Goodman, Charles Elmaraghy, Lauren O Bakaletz |
Journal | The Laryngoscope
(Laryngoscope)
Vol. 130
Issue 6
Pg. 1364-1371
(06 2020)
ISSN: 1531-4995 [Electronic] United States |
PMID | 31314141
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Copyright | © 2019 The American Laryngological, Rhinological and Otological Society, Inc. |
Chemical References |
- Antibodies, Bacterial
- Integration Host Factors
- DnaB Helicases
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Topics |
- Antibodies, Bacterial
(administration & dosage)
- Biofilms
(growth & development)
- Chronic Disease
- DnaB Helicases
(immunology)
- Haemophilus Infections
(prevention & control)
- Haemophilus influenzae
(immunology)
- Humans
- Integration Host Factors
(immunology)
- Paranasal Sinuses
(microbiology, surgery)
- Prostheses and Implants
(microbiology)
- Rhinitis
(microbiology, surgery)
- Sinusitis
(microbiology, surgery)
- Treatment Outcome
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