Abstract |
Chlorpyrifos (CPF) has become a mainly pollution in water environment. Micro-RNAs ( miRNAs) play an important part in the development of apoptosis and autophagy. However, the potential mechanism of CPF induced kidney toxicity and the roles of miRNAs are still unclear. To explore the underlying mechanism, the kidney of common carp exposed to different concentrations of CPF for 40 days was used as a research object. We found that CPF could damage the ultrastructure and function of kidney; and also caused antioxidant system disorder. CPF inhibited the mRNA level of miR-19a which improved AMP-activated protein kinase (AMPK). Furthermore, the detection of apoptosis and autophagy relative genes showed that the expressions of TSC complex subunit 2 (TSC2), light chain 3 (LC3), Dynein, tumor protein 53 (p53), Bcl-2 associated X protein (Bax), caspase-3 and caspase-9 were enhanced and the expressions of nechanistic target of rapamycin (mTOR), Ras homolog mTORC1 binding (Rheb) and B-cell lymphoma (Bcl-2) were reduced in dose-dependent way. Taken together, we conclude that CPF causes oxidative stress and miR-19a-AMPK axis disorder, thereby promotes apoptosis and autophagy in common carp kidney. Our study will provide theoretical basis for toxicology research and environmental protection of CPF.
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Authors | Qiaojian Zhang, Shufang Zheng, Shengchen Wang, Wei Wang, Houjuan Xing, Shiwen Xu |
Journal | Fish & shellfish immunology
(Fish Shellfish Immunol)
Vol. 93
Pg. 1093-1099
(Oct 2019)
ISSN: 1095-9947 [Electronic] England |
PMID | 31310849
(Publication Type: Journal Article)
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Copyright | Copyright © 2019 Elsevier Ltd. All rights reserved. |
Chemical References |
- Fish Proteins
- Insecticides
- MicroRNAs
- Water Pollutants, Chemical
- AMP-Activated Protein Kinases
- Chlorpyrifos
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Topics |
- AMP-Activated Protein Kinases
(genetics, metabolism)
- Animals
- Apoptosis
(drug effects)
- Autophagy
(drug effects)
- Carps
(genetics, immunology, physiology)
- Chlorpyrifos
(adverse effects)
- Dose-Response Relationship, Drug
- Fish Proteins
(genetics, metabolism)
- Gene Expression Regulation
(immunology)
- Insecticides
(adverse effects)
- Kidney
(drug effects, enzymology)
- MicroRNAs
(genetics, metabolism)
- Oxidative Stress
(drug effects)
- Random Allocation
- Water Pollutants, Chemical
(adverse effects)
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