Abstract | OBJECTIVE: It is essential to characterize underlying molecular mechanism associated with head and neck squamous cell carcinoma ( HNSCC) and identify promising therapeutic targets. Herein, we explored role of homeobox transcript antisense RNA (HOTAIR) in HNSCC to regulate stanniocalcin-2 (STC2) by sponging microRNA-206 (miR-206). METHODS:
HNSCC-related differentially expressed genes and regulation network amongst HOTAIR, miR-206 and STC2 were identified. Next, effect of HOTAIR on cell biological functions of HNSCC was identified after transfection of cells with HOTAIR overexpressed plasmids or siRNA against HOTAIR. PI3K/AKT signalling pathway-related gene expression was measured after miR-206 and STC2 were suppressed. Cell invasion, migration and proliferation were assessed. Finally, tumour growth was assessed to determine the effects of HOTAIR/miR-206/STC2 axis in vivo. RESULTS: HOTAIR specifically bound to miR-206 and miR-206 targeted STC2. Downregulated HOTAIR or upregulated miR-206 suppressed HNSCC cell proliferation, invasion and migration. miR-206 inhibited PI3K/AKT signalling pathway by down-regulating STC2. Besides, silenced HOTAIR or overexpressed miR-206 repressed the tumour growth of nude mice with HNSCC. CONCLUSION: HOTAIR regulated HNSCC cell biological functions by binding to miR-206 through STC2.
|
Authors | Tiancheng Li, Yao Qin, Zhen Zhen, Hong Shen, Tiechuan Cong, Erik Schiferle, Shuifang Xiao |
Journal | Cell proliferation
(Cell Prolif)
Vol. 52
Issue 5
Pg. e12651
(Sep 2019)
ISSN: 1365-2184 [Electronic] England |
PMID | 31297902
(Publication Type: Journal Article)
|
Copyright | © 2019 The Authors. Cell Proliferation Published by John Wiley & Sons Ltd. |
Chemical References |
- Glycoproteins
- HOTAIR long untranslated RNA, human
- Intercellular Signaling Peptides and Proteins
- MIRN206 microRNA, human
- MicroRNAs
- RNA, Long Noncoding
- RNA, Small Interfering
- STC2 protein, human
- Phosphatidylinositol 3-Kinases
- Proto-Oncogene Proteins c-akt
|
Topics |
- Animals
- Apoptosis
- Cell Line, Tumor
- Cell Movement
- Cell Proliferation
- Female
- Gene Expression Regulation, Neoplastic
- Glycoproteins
(antagonists & inhibitors, genetics, metabolism)
- Head and Neck Neoplasms
(metabolism, pathology)
- Humans
- Intercellular Signaling Peptides and Proteins
(genetics, metabolism)
- Mice
- Mice, Nude
- MicroRNAs
(antagonists & inhibitors, genetics, metabolism)
- Phosphatidylinositol 3-Kinases
(metabolism)
- Proto-Oncogene Proteins c-akt
(metabolism)
- RNA Interference
- RNA, Long Noncoding
(antagonists & inhibitors, genetics, metabolism)
- RNA, Small Interfering
(metabolism)
- Signal Transduction
- Squamous Cell Carcinoma of Head and Neck
(metabolism, pathology)
|