Abstract |
Cultures of JU56 cells were irradiated with 2.5 Gy X-rays and 16 h later the cultures were exposed to a moderately inhibitory dose of 1-beta-D-arabinofuranosylcytosine ( ara-C) or aphidicolin (APC) and to colcemid, for 2 h. The c-metaphases collected for examination had therefore been exposed to X-rays in G1 or early S, and to the repair inhibitors APC and ara-C during the latter half of G2. It was found that treatment of cells irradiated early in cell cycle, that is, in G1 and early S, with APC or ara-C in G2, (1) reduced the frequency of chromatid and chromosome exchanges below that of cells treated with X-rays alone, (2) produced no more chromatid breaks and gaps than were seen in unirradiated cells, (3) increased the number of chromosome fragments and gaps in a more than additive fashion, and (4) produced only an additive effect, by comparison with the effect of X-rays and drug given separately, on the total number of chromosomal aberrations.
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Authors | R C Moore, C Randell, M A Bender |
Journal | Mutation research
(Mutat Res)
Vol. 199
Issue 1
Pg. 229-33
(May 1988)
ISSN: 0027-5107 [Print] Netherlands |
PMID | 3129653
(Publication Type: Journal Article)
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Chemical References |
- Diterpenes
- Cytarabine
- Aphidicolin
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Topics |
- Animals
- Aphidicolin
- Cells, Cultured
- Chromosome Aberrations
- Cytarabine
(pharmacology)
- DNA Damage
- DNA Repair
(drug effects)
- Diterpenes
(pharmacology)
- Interphase
- Sister Chromatid Exchange
(drug effects)
- X-Rays
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