Apoptosis is involved in 2,2',4,4'- tetrabromodiphenyl
ether (PBDE-47)-induced developmental neurotoxicity. However, little is known about the role of autophagy, especially its relationship with apoptosis underlying such neurotoxic process. Methods: Using female Sprague-Dawley rats exposed to low-dose
PBDE-47 (0.1, 1.0 and 10 mg/kg/day) from pre-pregnancy until weaning of offspring to mimic human exposure, we investigated the effects of
PBDE-47 on autophagy and apoptosis in relation to
cognitive impairment of adult offspring rats. We also evaluated relationship between autophagy and apoptosis using neuroendocrine
pheochromocytoma (PC12) cells, a widely used neuron-like cell line for neuronal development. Results: In vivo, perinatal exposure to
PBDE-47 induced
memory deficits in adult rats. This is accompanied by hippocampal neuronal loss partly as a result of apoptosis, as evidenced by
caspase-3 activation and PARP cleavage. Further study identified that
PBDE-47 triggered autophagic vesicles accumulation, increased levels of
microtubule-associated protein 1 light chain 3 (LC3)-II, an essential
protein for autophagosomes formation, and autophagy substrate sequestosome 1 (SQSTM1/p62), but reduced levels of
autophagy-related protein (ATG) 7, a key
protein for autophagosomes elongation, suggestive of autophagy impairment. These findings were further demonstrated by an in vitro model of PBDE-47-treated PC12 cells. Mechanistically, autophagy alteration is more sensitive to
PBDE-47 treatment than apoptosis induction. Importantly, while stimulation of autophagy by the chemical inducer
rapamycin and adenovirus-mediated Atg7 overexpression aggravated PBDE-47-induced apoptosis and cell death, inhibition of autophagy by the chemical inhibitor
wortmannin and
siRNA knockdown of Atg7 reversed PBDE-47-produced detrimental outcomes. Interestingly, blockage of apoptosis by
caspase-3 inhibitor
Ac-DEVD-CHO ameliorated PBDE-47-exerted autophagy impairment and cell death, though in combination with autophagy inhibitor did not further promote cell survival. Conclusion: Our data suggest that autophagy impairment facilitates apoptosis, which, in turn, disrupts autophagy, ultimately resulting in cell death, and that autophagy may act as a promising therapeutic target for PBDE-47-induced developmental neurotoxicity.