Bee Pollen Extracts Modulate Serum Metabolism in Lipopolysaccharide-Induced Acute Lung Injury Mice with Anti-Inflammatory Effects.

Abstract

Bee pollen (BP) collected from different floras possesses various potential bioactivities, but the mechanism-related research on anti-inflammatory effects is limited. Here, three types of BP originating from Camellia sinensis L. (BP-Cs), Nelumbo nucifera Gaertn. (BP-Nn), and Brassica campestris L. (BP-Bc) were assessed using molecular and metabolomics methods to determine their anti-inflammatory effects. The differences in polyphenolic abundance of three types of BP extracts were determined by HPLC-DAD/Q-TOF-MS. In vitro anti-inflammatory effects of three BP extracts were evaluated in a lipopolysaccharide (LPS)-induced RAW 264.7 cells model. BP-Cs extract with the most abundant polyphenols was found to be the most effective in reducing inflammation by downregulating inflammatory-related genes expression and blocking the activation of MAPK and NF-κB signaling pathways. Polyphenol-rich BP-Cs was further evaluated for their in vivo anti-inflammatory effect in a LPS-induced acute lung injury mouse model. An UPLC-Q-TOF/MS-based metabolomics approach was applied to analyze metabolite changes in mouse serum. Weshowed that the pretreated BP-Cs extract alleviated inflammation and regulated glycerophospholipid metabolism significantly. Our findings provide a foundation for developing and justifying BP as a potential anti-inflammatory ingredient in functional foods or nutraceutical formulations.

• Authors: Qiangqiang Li, Minghui Sun, Zhengrui Wan, Junshi Liang, Mirko Betti, Yuliya Hrynets, Xiaofeng Xue, Liming Wu, Kai Wang
• Journal: Journal of agricultural and food chemistry (J Agric Food Chem) Vol. 67 Issue 28 Pg. 7855-7868 (Jul 17 2019) ISSN: 1520-5118 [Electronic] United States
• PMID: 31274310 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Lipopolysaccharides
• NF-kappa B
• Plant Extracts
• Polyphenols
• Mitogen-Activated Protein Kinase Kinases

Topics

• Acute Lung Injury (drug therapy, genetics, immunology)
• Animals
• Anti-Inflammatory Agents (administration & dosage, chemistry)
• Bees
• Brassica (chemistry)
• Camellia sinensis (chemistry)
• Chromatography, High Pressure Liquid
• Humans
• Lipopolysaccharides (adverse effects)
• Macrophages (drug effects, immunology)
• Male
• Mass Spectrometry
• Mice
• Mice, Inbred ICR
• Mitogen-Activated Protein Kinase Kinases (genetics, immunology)
• NF-kappa B (genetics, immunology)
• Nelumbo (chemistry)
• Plant Extracts (administration & dosage, chemistry)
• Pollen (chemistry)
• Polyphenols (administration & dosage, chemistry)
• RAW 264.7 Cells