Mesothelin, Calretinin, and Megakaryocyte Potentiating Factor as Biomarkers of Malignant Pleural Mesothelioma.

Abstract

PURPOSE:

Malignant pleural mesothelioma (MPM) is a highly lethal cancer caused by exposure to asbestos. Currently, the diagnosis is a challenge, carried out by means of invasive methods of limited sensitivity. This is a case-control study to evaluate the individual and combined performance of minimally invasive biomarkers for the diagnosis of MPM.

METHOD:

A study of 166 incident cases of MPM and 378 population controls of Mestizo-Mexican ethnicity was conducted. Mesothelin, calretinin, and megakaryocyte potentiating factor (MPF) were quantified in plasma by ELISA. The samples were collected from 2011 to 2016.

RESULTS:

Based on ROC analysis and a preset specificity of 95%, the combination of the three biomarkers reached an AUC of 0.944 and a sensitivity of 82% in men. In women, an AUC of 0.937 and a sensitivity of 87% were reached. In nonconditional logistic regression models, the adjusted ORs in men were 7.92 (95% CI 3.02-20.78) for mesothelin, 20.44 (95% CI 8.90-46.94) for calretinin, and 4.37 (95% CI 1.60-11.94) for MPF. The ORs for women were 28.89 (95% CI 7.32-113.99), 17.89 (95% CI 3.93-81.49), and 2.77 (95% CI 0.47-16.21), respectively.

CONCLUSIONS:

To our knowledge, this is the first study evaluating a combination of mesothelin, calretinin, and MPF, and demonstrating a sex effect for calretinin. The biomarker panel showed a good performance in a Mestizo-Mexican population, with high sensitivity and specificity for the diagnosis of MPM.

• Authors: Carmina Jiménez-Ramírez, Swaantje Casjens, Cuauhtémoc Arturo Juárez-Pérez, Irina Raiko, Luz M Del Razo, Dirk Taeger, Emma S Calderón-Aranda, Hans-Peter Rihs, Leonor Concepción Acosta-Saavedra, Daniel Gilbert Weber, Alejandro Cabello-López, Beate Pesch, María Dolores Ochoa-Vázquez, Katarzyna Burek, Luis Torre-Bouscoulet, José Rogelio Pérez-Padilla, Erik Marco García-Bazan, Thomas Brüning, Georg Johnen, Guadalupe Aguilar-Madrid
• Journal: Lung (Lung) Vol. 197 Issue 5 Pg. 641-649 (10 2019) ISSN: 1432-1750 [Electronic] United States
• PMID: 31267149 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• CALB2 protein, human
• Calbindin 2
• GPI-Linked Proteins
• MSLN protein, human
• Mesothelin

Topics

• Aged
• Biomarkers, Tumor (blood)
• Calbindin 2 (blood)
• Enzyme-Linked Immunosorbent Assay
• Female
• GPI-Linked Proteins (blood)
• Humans
• Incidence
• Lung Neoplasms (blood, diagnosis, epidemiology)
• Male
• Mesothelin
• Mesothelioma (blood, diagnosis, epidemiology)
• Mesothelioma, Malignant
• Mexico (epidemiology)
• Middle Aged
• Pleural Neoplasms (blood, diagnosis, epidemiology)
• Predictive Value of Tests
• Prognosis
• Risk Assessment
• Risk Factors
• Sex Factors