Abstract |
Tumor microenvironment and metabolic activity in gliomas are the important biomarkers to evaluate the progression of gliomas. Many evidences have suggested that the targeting of metabolic activity and tumor microenvironment simultaneously can be more effective to take the tumor therapy. Therefore, the noninvasive, accurate assessment of tumor microenvironment and metabolic activity is quite important in clinical practice. Multiphoton microscopy (MPM), based on two-photon-excited fluorescence and second harmonic generation was performed on unstained glioma tissues. With our combined image analysis approaches, our research findings indicate that MPM is able to qualitatively and quantitatively describe the microenvironment characteristics in gliomas, such as collage deposition in extracellular matrix, lymphocyte infiltration and tumor angiogenesis, etc. Meanwhile, the metabolic activity can also be quantitatively evaluated by optical redox ratio, NADH and FAD intensity. With the microendoscope and fiberscope are portable, MPM technique can be used to perform in-vivo studies and clinical examinations in gliomas.
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Authors | Na Fang, Zanyi Wu, Xingfu Wang, Yuanxiang Lin, Lianhuang Li, Zufang Huang, Yupeng Chen, Xianying Zheng, Shanshan Cai, Haohua Tu, Dezhi Kang, Jianxin Chen |
Journal | Journal of biophotonics
(J Biophotonics)
Vol. 12
Issue 10
Pg. e201900136
(10 2019)
ISSN: 1864-0648 [Electronic] Germany |
PMID | 31251837
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Topics |
- Brain Neoplasms
(diagnostic imaging, immunology, metabolism, pathology)
- Glioma
(diagnostic imaging, immunology, metabolism, pathology)
- Humans
- Lymphocytes
(immunology)
- Microscopy, Fluorescence, Multiphoton
- Neovascularization, Pathologic
(diagnostic imaging)
- Tumor Microenvironment
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