Performance evaluation of a line blot assay system for detection of anti-PM-Scl antibody in Japanese patients with systemic sclerosis.

Abstract

OBJECTIVE:

To evaluate the performance of a line blot (LB) assay for identifying anti-PM-Scl antibody (Ab).

METHODS:

We screened 56 serum samples from systemic sclerosis patients using a protein immunoprecipitation (IP) assay and an LB assay (Systemic Sclerosis Profile Euroline® Blot test kit) to detect anti-PM-Scl Ab. We compared the results obtained by the IP and LB assays.

RESULTS:

Among the 56 serum samples of SSc patients, 9 sera were positive for anti-PM-Scl75 Ab and 1 for anti-PM-Scl100 Ab by the LB assay. The protein IP assay revealed that none of the samples precipitated 75 or 100 kDa proteins identical to the anti-PM-Scl Ab reference serum, regardless of the positive or negative results obtained in the LB assay. Therefore, the false-positive rates for both anti-PM-Scl75 Ab and anti-PM-Scl100 Ab by the LB assay were 100%.

CONCLUSION:

Detection of anti-PM-Scl Ab assessed with a commercial LB assay requires extreme caution since it may yield false-positive data.

• Authors: Yasuhito Hamaguchi, Masataka Kuwana, Kazuhiko Takehara
• Journal: International journal of rheumatic diseases (Int J Rheum Dis) Vol. 22 Issue 9 Pg. 1746-1751 (Sep 2019) ISSN: 1756-185X [Electronic] England
• PMID: 31245910 (Publication Type: Comparative Study, Evaluation Study, Journal Article)
• Copyright: © 2019 Asia Pacific League of Associations for Rheumatology and John Wiley & Sons Australia, Ltd.

Chemical References

• Autoantibodies
• Biomarkers
• EXOSC9 protein, human
• RNA-Binding Proteins
• Exoribonucleases
• Exosome Multienzyme Ribonuclease Complex
• EXOSC10 protein, human

Topics

• Autoantibodies (blood)
• Biomarkers (blood)
• Exoribonucleases (immunology)
• Exosome Multienzyme Ribonuclease Complex (immunology)
• False Positive Reactions
• Humans
• Immunologic Tests
• Japan
• Predictive Value of Tests
• RNA-Binding Proteins (immunology)
• Reproducibility of Results
• Scleroderma, Systemic (blood, diagnosis, immunology)