Abstract | BACKGROUND: METHODS: The viability of BGC-823 cells was detected by MTT assay. The apoptosis and mitochondrial membrane potential (ΔΨm) of BGC-823 cells induced by costunolide were analyzed by flow cytometry. The inhibiton of costunolide on human gastric adenocarcinoma was estimated in xenografts in nude mice. Apoptosis related proteins and genes were detected by Western blot and Q-PCR. RESULTS: CONCLUSIONS: Collectively, our results suggested that costunolide induced mitochondria-mediated apoptosis in human gastric adenocarcinoma BGC-823 cells and could be the candidate drug against GC in clinical practice.
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Authors | Zhanpeng Yan, Tingting Xu, Zhentao An, Ying Hu, Wanzhen Chen, Jinxia Ma, Changle Shao, Fangshi Zhu |
Journal | BMC complementary and alternative medicine
(BMC Complement Altern Med)
Vol. 19
Issue 1
Pg. 151
(Jun 26 2019)
ISSN: 1472-6882 [Electronic] England |
PMID | 31242894
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents, Phytogenic
- Proto-Oncogene Proteins c-bcl-2
- Sesquiterpenes
- costunolide
- Poly(ADP-ribose) Polymerases
- Caspases
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Topics |
- Adenocarcinoma
(drug therapy, genetics, metabolism, physiopathology)
- Animals
- Antineoplastic Agents, Phytogenic
(administration & dosage)
- Apoptosis
(drug effects)
- Caspases
(genetics, metabolism)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Female
- Humans
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- Mitochondria
(drug effects, metabolism)
- Poly(ADP-ribose) Polymerases
(genetics, metabolism)
- Proto-Oncogene Proteins c-bcl-2
(genetics, metabolism)
- Sesquiterpenes
(administration & dosage)
- Stomach Neoplasms
(drug therapy, genetics, metabolism, physiopathology)
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