Long noncoding RNA PXN-AS1-L promotes the malignancy of nasopharyngeal carcinoma cells via upregulation of SAPCD2.

Abstract

Accumulating evidences highlight the critical roles of long noncoding RNAs (lncRNAs) in a variety of cancers. LncRNA PXN-AS1-L was previously shown to exert oncogenic roles in hepatocellular carcinoma. However, the expression, role, and molecular mechanism of PXN-AS1-L in nasopharyngeal carcinoma (NPC) malignancy remain unknown. Here, we determined that PXN-AS1-L is upregulated in NPC tissues and cell lines. Increased expression of PXN-AS1-L predicts worse prognosis of NPC patients. PXN-AS1-L overexpression promotes NPC cell proliferation, migration, and invasion in vitro, and NPC tumor growth in vivo. PXN-AS1-L silencing suppresses NPC cell proliferation, migration, and invasion in vitro. Mechanistically, PXN-AS1-L directly interacts with SAPCD2 mRNA 3'-untranslated region, prevents the binding of microRNAs-AGO silencing complex to SAPCD2 mRNA, and upregulates the mRNA and protein level of SAPCD2. SAPCD2 is also increased in NPC tissues. The expression of SAPCD2 is significantly positively associated with that of PXN-AS1-L in NPC tissues. Gain-of-function and loss-of-function experiments demonstrated that SAPCD2 also promotes NPC cell proliferation, migration, and invasion. Furthermore, depletion of SAPCD2 significantly reverses the roles of PXN-AS1-L in promoting NPC cell proliferation, migration, and invasion in vitro, and NPC tumor growth in vivo. In conclusion, lncRNA PXN-AS1-L is upregulated in NPC and promoted NPC malignancy by upregulating SAPCD2 via direct RNA-RNA interaction.

• Authors: Xiaodong Jia, Po Niu, Cuncun Xie, Hongjian Liu
• Journal: Cancer medicine (Cancer Med) Vol. 8 Issue 9 Pg. 4278-4291 (08 2019) ISSN: 2045-7634 [Electronic] United States
• PMID: 31173488 (Publication Type: Journal Article)
• Copyright: © 2019 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.

Chemical References

• 3' Untranslated Regions
• Nuclear Proteins
• PXN-AS1 non-coding RNA, human
• RNA, Long Noncoding
• SAPCD2 protein, human

Topics

• 3' Untranslated Regions
• Animals
• Cell Line, Tumor
• Cell Movement
• Cell Proliferation
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Male
• Mice
• Nasopharyngeal Carcinoma (genetics, pathology)
• Nasopharyngeal Neoplasms (genetics, pathology)
• Neoplasm Transplantation
• Nuclear Proteins (genetics)
• Prognosis
• RNA, Long Noncoding (genetics)
• Survival Analysis
• Up-Regulation