The present study aimed to investigate the expression of
pyrroline-5-carboxylate reductase 1 (P5CR1)
protein in
lung adenocarcinoma and paracancerous tissues and to explore the effect of silencing the encoding gene PYCR1 on the proliferation, migration, invasion, and
cisplatin sensitivity in
lung adenocarcinoma cells, thereby providing a novel therapeutic target for the treatment of the disease. Immunohistochemistry staining was used to detect the P5CR1
protein expression in
lung adenocarcinoma and paracancerous tissues, and statistical analysis evaluated the correlation between P5CR1
protein expression and gender, age, tissue part, or pathological grade. The CCK8 assay was performed to detect the proliferation and
cisplatin sensitivity, while the effect of PYCR1 on the migration and invasion of
lung adenocarcinoma cells was detected by scratch test and transwell chamber assay. The findings demonstrated that the P5CR1
protein expression was significantly elevated in
lung adenocarcinoma tissues and correlated with the pathological grade, whereas no significant correlation was established between the
protein expression and gender, age, or tissue part. Furthermore, after PYCR1 gene silencing, the proliferation and invasion were significantly suppressed, while the sensitivity to
cisplatin was significantly enhanced. Therefore, it can be speculated that the PYCR1 gene affects the
biological behavior of
lung adenocarcinoma and
cisplatin resistance, serving as a potential therapeutic target for
lung adenocarcinoma.