Panax notoginsenoside Rb1 Restores the Neurotrophic Imbalance Following Photothrombotic Stroke in Rats.

Abstract

Mature brain-derived neurotrophic factor (mBDNF) has neuroprotection in cerebral ischemia. Conversely, the precursor of brain-derived neurotrophic factor (proBDNF) has the opposite function to its mature form, inducing apoptosis. However, whether the neuroprotection of Panax notoginsenoside Rb1 (PNS-Rb1) on ischemic stroke is due to, at least partially, its modulation of suppressing proBDNF/P75NTR/sortilin or upregulation of mBDNF is not clear. To test this hypothesis, rats induced by photothrombotic stroke were treated with PNS-Rb1 100 mg/kg or nimodipine 1 mg/kg twice a day until 3, 7, and 14 days. Our data indicate that PNS-Rb1 significantly reduced cerebral infarction rate, proBDNF/P75NTR/sortilin, and plasminogen activator inhibitor-1 (PAI-1) protein levels, and improved sensorimotor dysfunctions induced by ischemic stroke, upregulation of BDNF/TrkB levels, and its processing enzymes (tissue plasminogen activator, tPA) in a time-dependent manner. Taken together, our findings indicate that the improvement of sensorimotor dysfunctions by PNS-Rb1 following ischemic stroke is made, at least partially, by activating the BDNF/TrkB and inhibiting proBDNF/sortilin/P75NTR.

• Authors: Chun-Yan Yang, Jian-Yu Yang, Yun-Xia Xiong, Xue-Feng Zhuang, Hui Su, Sheng Hu, Jia-Qing Ma, Xin-Fu Zhou, Hai-Yun Luo, Jun Sun
• Journal: Neurotoxicity research (Neurotox Res) Vol. 36 Issue 3 Pg. 441-451 (Oct 2019) ISSN: 1476-3524 [Electronic] United States
• PMID: 31129909 (Publication Type: Journal Article)

Chemical References

• Adaptor Proteins, Vesicular Transport
• Brain-Derived Neurotrophic Factor
• Nerve Tissue Proteins
• Neuroprotective Agents
• Protein Precursors
• Receptors, Growth Factor
• Receptors, Nerve Growth Factor
• Saponins
• brain-derived neurotrophic factor precursor
• saponin Rb1, Panax notoginsenoside
• Ngfr protein, rat
• Ntrk2 protein, rat
• Receptor, trkB
• sortilin

Topics

• Adaptor Proteins, Vesicular Transport (metabolism)
• Animals
• Blotting, Western
• Brain (metabolism)
• Brain-Derived Neurotrophic Factor (metabolism)
• Disease Models, Animal
• Enzyme-Linked Immunosorbent Assay
• Male
• Motor Skills
• Nerve Tissue Proteins
• Neuroprotective Agents (therapeutic use)
• Panax
• Protein Precursors (metabolism)
• Rats
• Rats, Sprague-Dawley
• Receptor, trkB (metabolism)
• Receptors, Growth Factor
• Receptors, Nerve Growth Factor (metabolism)
• Saponins (therapeutic use)
• Stroke (drug therapy, metabolism)